Zinc fixation for flow cytometry analysis of intracellular and surface epitopes, DNA content, and cell proliferation.
Zinc fixation for flow cytometry analysis of intracellular and surface epitopes, DNA content, and cell proliferation.
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锌固定用于流式细胞术分析细胞内和表面表位、DNA 含量和细胞增殖。
DOI：
10.1002/0471142956.cy0740s57
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发表时间：
2011
影响因子：
--
通讯作者：
Jensen,UffeBirk
中科院分区：
文献类型：
--
作者：
Christensen,Rikke;Owens,DavidM;Thomsen,Anette;Pedersen,Søren;Jensen,UffeBirk
基于锌盐的固定（ZBF）是一种简单、经济、无害的细胞悬液固定方法，可保留所有细胞结构，并在同一细胞样品中使用胸苷类似物EdU进行表面和细胞内蛋白质、DNA含量谱和脉冲标记的流式细胞术分析。如通过流式细胞术测量的，ZBF在表面表位标记和前向和侧向光散射参数的保存方面与甲醛表现得同样好。DNA保持在高分子量，提高了定量，并允许随后的定量PCR分析。最后，ZBF处理允许标记细胞的长期储存，这些参数几乎没有变化。方案细胞。57：7.40.1 - 7.40.9© 2011年由John Wiley & Sons，Inc.
Zinc salt‐based fixation (ZBF) is a simple, cost‐effective, and nonhazardous fixation method for cell suspensions that preserves all cellular structures and enables flow cytometric analysis of both surface and intracellular proteins, DNA content profiles, and pulse‐labeling using the thymidine analog EdU in the same cell sample. ZBF performs equally well to formaldehyde in the preservation of surface epitope labeling and forward and side light scatter parameters, as measured by flow cytometry. DNA is maintained at high molecular weight, improving the quantification and allowing subsequent quantitative PCR analysis. Finally, ZBF treatment allows for long‐term storage of labeled cells with little change in these parameters.Curr. Protoc. Cytom. 57:7.40.1‐7.40.9. © 2011 by John Wiley & Sons, Inc.