Effects of lifelong spontaneous exercise on the M1/M2 macrophage polarization ratio and gene expression in adipose tissue of super-aged mice
Effects of lifelong spontaneous exercise on the M1/M2 macrophage polarization ratio and gene expression in adipose tissue of super-aged mice
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DOI：
10.1016/j.exger.2020.111091
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发表时间：
2020-11-01
影响因子：
3.9
通讯作者：
Kim, Ji-Seok
中科院分区：
文献类型：
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作者：
Baek, Kyung-Wan;Lee, Da-In;Kim, Ji-Seok
在脂肪组织（AT）中，M1巨噬细胞(M1empty set)/M2巨噬细胞（M2empty set）极化比的增加可能是衰老过程中炎症反应增强的危险因素，也可能增加慢性疾病的风险，从而缩短寿命，或至少缩短“健康”寿命。本研究的目的是分析和比较老年动物和对照组进行终身自发轮跑时AT M1empty set/M2empty set极化比。流式细胞术分析显示，终生自发运动（LSE）后，巨噬细胞AT比值显示M2empty set极化，与年龄无关。然而，对于Icam1和Tnf， qPCR分析显示在幼鼠中基因表达没有差异；Arg1在Young-EXE（运动组）小鼠中的表达高于Young-CON（对照组）小鼠(p
In the adipose tissue (AT), an increase in the M1 macrophage (M1empty set)/M2 macrophage (M2empty set) polarization ratio can be a risk factor enhancing the inflammatory response during aging, as well as increasing the risk of chronic disease, thereby reducing lifespan, or at least reducing "healthy" lifespan. The purpose of this study was to analyze and compare the AT M1empty set/M2empty set polarization ratio at the final lifespan stage in aged and control animals performing lifelong spontaneous wheel running. Based on flow cytometric analysis, the AT ratio of macrophages revealed M2empty set polarization following lifelong spontaneous exercise (LSE) regardless of age. However, for Icam1 and Tnf, the qPCR analysis showed no difference in gene expressions in young mice; Arg1 expression was higher in Young-EXE (exercising) than in Young-CON (control) mice (p