Diversity of murine norovirus in wild-rodent populations: species-specific associations suggest an ancient divergence.
Diversity of murine norovirus in wild-rodent populations: species-specific associations suggest an ancient divergence.
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野生啮齿动物种群中鼠诺如病毒的多样性：物种特异性关联表明存在古老的分歧。
DOI：
10.1099/vir.0.036392-0
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
P. Simmonds
中科院分区：
文献类型：
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作者：
Donald B. Smith;Nora McFadden;R. Blundell;A. Meredith;P. Simmonds
对野生啮齿动物种群的调查显示，野生家鼠小家鼠中存在鼠诺如病毒 (MNV)，且具有多样性。这种病毒在基因上与感染小鼠的 MNV 以及先前描述的在实验室小鼠中传播的变种相似。在野生小鼠群体中检测到 MNV 表明，实验室小鼠和展示小鼠（实验小鼠的来源）的 MNV 感染源自与野生小鼠祖细胞的接触或起源。该调查还发现，木鼠（Apodemus sylvaticus）经常感染 MNV 基因不同的变体。这些病毒与之前描述的 MNV 变种不同，其完整基因组序列的差异为 22-23%，而小家鼠衍生株之间的差异最大为 13%。与其他诺如病毒的比较表明，黑线姬鼠 MNV 与基因组 V 中的 MNV 属于同一组，并且具有相同的整体基因组组织、ORF 1-3 编码的蛋白质的预测长度以及编码 MNV ORF4 同源物的 VP1 中存在保守的替代阅读框。不同的姬鼠 MNV 分离株与 MNV 分离株一样变异，并显示出分离株间重组的证据。我们对野生种群中 MNV 变体的物种特异性关联的观察表明，鼠诺如病毒具有古老的起源，这一特征可能与其他诺如病毒基因组共有。
A survey of wild-rodent populations has revealed that murine norovirus (MNV) is present and diverse in wild-house mice Mus musculus. This virus is genetically similar to MNV infecting show mice and previously described variants circulating in laboratory mice. The detection of MNV in wild-mouse populations suggests that MNV infection of laboratory mice and show mice (from which laboratory mice are derived) derives from contact with or their origins from wild-mouse progenitors. The survey additionally identified frequent infection of wood mice (Apodemus sylvaticus) with genetically divergent variants of MNV. These viruses are distinct from previously described MNV variants, differing by 22-23 % over the complete genome sequence compared with a maximum of 13 % between M. musculus-derived strains. Comparison with other noroviruses reveals that the Apodemus MNV groups with MNV in genogroup V and shares the same overall genome organization, predicted lengths of proteins encoded by ORFs 1-3 and the existence of a conserved alternative reading frame in VP1 encoding a homologue of the MNV ORF4. Different Apodemus MNV isolates were as variable as MNV isolates and showed evidence for inter-isolate recombination. Our observation of species-specific associations of MNV variants in wild populations suggests that murine noroviruses have an ancient origin, a feature that they may share with other norovirus genogroups.