Anthropogenic and climate-induced change favors toxic cyanobacteria blooms: Evidence from monitoring a highly eutrophic, urban Mediterranean lake
Anthropogenic and climate-induced change favors toxic cyanobacteria blooms: Evidence from monitoring a highly eutrophic, urban Mediterranean lake
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DOI：
10.1016/j.hal.2014.09.002
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发表时间：
2014-10-01
期刊：
影响因子：
6.6
通讯作者：
Leonardos, Ioannis
中科院分区：
文献类型：
--
作者：
Gkelis, Spyros;Papadimitriou, Theodoti;Leonardos, Ioannis
蓝藻水华（或CyanoHABs）是水生环境中最明显的水生微生物危害之一，主要是由于产生有害的次级代谢产物，称为蓝藻毒素。在本文中，我们使用多种方法评估了地中海浅水高度富营养化湖泊（希腊西北部帕姆沃蒂斯湖）中蓝细菌水华与关键环境参数的关系。显微镜（蓝藻识别），免疫学（ELISA），分子技术（PCR）结合物理化学参数被用来监测蓝藻水华和相关的蓝藻毒素生产每月14个月。蓝藻是浮游植物的主要组成部分，在整个研究期间占浮游植物总丰度的75%以上，优势种属于微囊藻，鱼腥藻和束丝藻。微囊藻毒素（MCs）检测到全年在所有采样站的浓度范围从0.16至27微克L-1，表明有毒鱼腥藻和微囊藻菌株是持久性和占主导地位。石房蛤毒素（STXs）中发现的两个样品（浓度1.3和2.1 μ g L-1）在温暖的时期，在这里首次报道。总MC浓度与温度、硝态氮、氨氮和可溶性活性磷呈正相关，表明高温和富营养化条件促进了MC生产基因型的生长。我们还研究了微囊藻菌株的特定生长速率，其在30 ℃时达到最大值。我们的数据，结合长期的数据比较的关键湖沼学特征，跨越25年的时间，表明水温和营养负荷可能会协同作用，以促进蓝藻的优势和持久性在潘沃蒂斯湖。(C)2014爱思唯尔有限公司版权所有。
Cyanobacteria Harmful Algal Blooms (or CyanoHABs) represent one of the most conspicuous waterborne microbial hazards in aquatic environments mostly due to the production of harmful secondary metabolites, known as cyanotoxins. In this paper we assessed cyanobacteria blooms in a shallow, Mediterranean, highly eutrophic lake (Lake Pamvotis, NW Greece) in relation to critical environmental parameters using a multi-approach methodology. Microscopic (identification of cyanobacteria), immunological (ELISA), and molecular techniques (PCR) combined with physico-chemical parameters were used to monitor cyanobacteria blooms and the associated cyanotoxin production for 14 months on a monthly basis. Cyanobacteria were the main phytoplankton component, representing more than 75% of the total phytoplankton abundance throughout the study period; dominant species belonged to Microcystis, Anabaena and Aphanizomenon. Microcystins (MCs) were detected throughout the year in all sampling stations in concentrations ranging from 0.16 to 27 mu g L-1, indicating that toxic Anabaena and Microcystis strains are persistent and dominant. Saxitoxins (STXs) found in two samples (concentrations 1.3 and 2.1 mu g L-1) in the warm period, are reported here for the first time. The total MC concentration was positively correlated with temperature, nitrate nitrogen, ammonia nitrogen, and soluble reactive phosphorus, suggesting that high-temperatures and eutrophic conditions promote the growth of MC-producing genotypes. We also investigated a Micro cystis strain specific growth rate, which reached its maximum at 30 degrees C. Our data, combined with long-term data comparison of key limnological features, spanning a 25-year period, suggest that water temperature and nutrient loads may act synergistically to promote cyanobacterial dominance and persistence in Lake Pamvotis. (C) 2014 Elsevier B.V. All rights reserved.