Lipid requirement of the vanadate effect on the binding of calcium and ATP to the calcium transport ATPase of the sarcoplasmic reticulum.
Lipid requirement of the vanadate effect on the binding of calcium and ATP to the calcium transport ATPase of the sarcoplasmic reticulum.
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钒酸盐的脂质需求影响钙和 ATP 与肌浆网钙转运 ATP 酶的结合。
DOI：
10.1111/j.1432-1033.1985.tb08647.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
W. Hasselbach
中科院分区：
文献类型：
--
作者：
P. Medda;W. Hasselbach
肌浆网钙转运ATP酶的脂质剥夺既不影响酶对ATP的亲和力，也不影响钙的亲和力。相比之下，钒酸盐结合几乎完全消除。以0.3 mg/mg蛋白质的比例，油酸的脂质替代完全重新激活钙依赖性ATP水解，恢复钒酸盐结合。同时，钒酸盐和钙或ATP和ADP之间的相互作用分别恢复。钒酸盐诱导的酶的ATP结合位点的消失以及其高亲和力的钙结合位点遵循相同的时间过程。相反，钒酸盐被钙的置换与ADP结合的恢复平行进行。在脂质恢复制剂以及在天然膜钒酸盐引起的外部高亲和力的消失，同时出现内部低亲和力的钙结合位点。
Lipid deprivation of the sarcoplasmic reticulum calcium-transport ATPase neither affects the enzyme's affinity for ATP nor that of calcium. In contrast, vanadate binding is almost completely abolished. Lipid substitution by oleic acid which at a ratio of 0.3 mg/mg protein completely reactivates the calcium-dependent ATP hydrolysis restores vanadate binding. Concomitantly the mutual interactions between vanadate and calcium or ATP and ADP, respectively are restored. The vanadate-induced disappearance of the enzyme's ATP binding sites as well as its high-affinity binding sites for calcium follow the same time course. Conversely, the displacement of vanadate by calcium proceeds in parallel with the recovery of ADP binding. In lipid-restituted preparations as well as in native membranes vanadate induces the disappearance of external high-affinity and simultaneously the appearance of internal low-affinity calcium binding sites.
肌浆网 ATP 酶中 H 、 Ca2 和 Pi（或钒酸盐）位点的相互依赖性。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Inesi,G;Lewis,D;Murphy,AJ
通讯作者：
Murphy,AJ