Motoneurons and oligodendrocytes are sequentially generated from neural stem cells but do not appear to share common lineage-restricted progenitors in vivo
Motoneurons and oligodendrocytes are sequentially generated from neural stem cells but do not appear to share common lineage-restricted progenitors in vivo
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DOI：
10.1242/dev.02236
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发表时间：
2006-02-01
期刊：
影响因子：
4.6
通讯作者：
Capecchi, MR
中科院分区：
文献类型：
--
作者：
Wu, S;Wu, YY;Capecchi, MR
寡基因表达被认为是运动神经元和少突胶质细胞共同祖细胞的标志。为了进一步剖析运动神经元和少突胶质细胞之间的体内谱系关系，我们使用了一种条件细胞消融方法来杀死表达oligo的细胞。虽然分化的运动神经元和少突胶质细胞被消除，但我们的消融研究显示它们的前体持续产生并随后死亡。最值得注意的是，在小鼠发育的第12天，在所有的运动神经元前体被杀死之后，形成了正常数量的少突胶质细胞前体。本文提供的数据支持一个序列模型，其中运动神经元和少突胶质细胞前体在体内由神经上皮干细胞依次产生，但不具有共同的谱系限制祖细胞。
Olig gene expression is proposed to mark the common progenitors of motoneurons and oligodendrocytes. In an attempt to further dissect the in vivo lineage relationships between motoneurons and oligodendrocytes, we used a conditional cell-ablation approach to kill Olig-expressing cells. Although differentiated motoneurons and oligodendrocytes were eliminated, our ablation study revealed a continuous generation and subsequent death of their precursors. Most remarkably, a normal number of oligodendrocyte precursors are formed at day 12 of mouse development, after all motoneuron precursors have been killed. The data presented herein supports a sequential model in which motoneuron and oligodendrocyte precursors are sequentially generated in vivo from neuroepithelial stem cells, but do not share a common lineage-restricted progenitor.