Viral bronchiolitis during early life induces increased numbers of bronchiolar mast cells and airway hyperresponsiveness.
Viral bronchiolitis during early life induces increased numbers of bronchiolar mast cells and airway hyperresponsiveness.
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发表时间：
1990-10
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影响因子：
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通讯作者：
William L. Castleman;R. Sorkness;R. Lemanske;P. Mcallister
中科院分区：
文献类型：
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作者：
William L. Castleman;R. Sorkness;R. Lemanske;P. Mcallister
本研究的目的是确定仙台病毒诱导的细支气管肥大细胞增加的动力学，并确定病毒诱导的细支气管肥大细胞增加是否与气道对乙酰甲胆碱的反应性增加以及对抗原刺激的过敏性炎症反应改变有关。在病毒或假接种后 7、15、30、60 和 90 天，使用形态测量技术测量远交 CD (Crl:CDBR) 大鼠肺内气道中的肥大细胞密度。接种病毒后30、60和90天，接种病毒的大鼠细支气管肥大细胞密度高于对照大鼠（P小于0.01），但在接种后7和15天则没有差异。接种病毒后30天，大鼠肺肥大细胞总数增加。病毒接种后 42 天，大鼠对雾化甲胆碱浓度的敏感性增加了三倍多，这会刺激呼吸阻力增加 50%。对卵清蛋白敏感的病毒接种大鼠的肺中性粒细胞增加了 10 倍以上（P 小于 0.02），在卵清蛋白气雾剂攻击后 4 小时通过支气管肺泡灌洗恢复。此时，接种病毒的大鼠细支气管壁中的中性粒细胞密度也高于暴露于过敏原的对照大鼠。结果表明，仙台病毒在接种后30至90天内有时会诱导细支气管肥大细胞数量增加，并且肥大细胞增加与气道对乙酰甲胆碱的高反应性和加剧的过敏性气道炎症反应有关。
The objectives of this study were to determine the kinetics of Sendai virus-induced increases in bronchiolar mast cells and to determine whether virus-induced increases in bronchiolar mast cells were associated with increased airway responsiveness to methacholine and with altered allergic inflammatory responses to antigen stimulation. Mast cell density in intrapulmonary airways was measured in outbred CD (Crl:CDBR) rats by use of morphometric techniques at 7, 15, 30, 60, and 90 days after viral or sham inoculation. Density of bronchiolar mast cells was higher in virus-inoculated rats than in control rats at 30, 60, and 90 days after inoculation (P less than 0.01), but not at 7 or 15 days after inoculation. Total pulmonary mast cell numbers were increased in virus-inoculated rats at 30 days after inoculation. Rats at 42 days after viral inoculation had over a threefold increase in sensitivity to the concentration of nebulized metbacholine that would stimulate a 50% increase in respiratory resistance. Virus-inoculated rats sensitized to ovalbumin had over a 10-fold increase (P less than 0.02) in pulmonary neutrophils that were recovered by bronchoalveolar lavage at 4 hours after ovalbumin aerosol challenge. Virus-inoculated rats at this time also had higher densities of neutrophils in bronchiolar walls than allergen-exposed control rats. The results indicate that Sendai virus induces increases in numbers of bronchiolar mast cells at times from 30 to 90 days after inoculation, and that mast cell increases are associated with airway hyperresponsiveness to methacholine and heightened allergic airway inflammatory reactions.