Intraspecific Variability in Root Traits and Edaphic Conditions Influence Soil Microbiomes Across 12 Switchgrass Cultivars
Intraspecific Variability in Root Traits and Edaphic Conditions Influence Soil Microbiomes Across 12 Switchgrass Cultivars
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DOI：
10.1094/pbiomes-12-19-0069-fi
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
Tayler C. Ulbrich;M. Friesen;Sarah S. Roley;L. Tiemann;S. Evans
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作者：
Tayler C. Ulbrich;M. Friesen;Sarah S. Roley;L. Tiemann;S. Evans
微生物群落帮助植物获取营养和承受压力。一些微生物组是特定于植物基因型的，因此可能有助于植物生长的种内差异，并成为植物育种的一个有希望的目标。柳枝稷（Panicum virgatum）是一种潜在的生物能源作物，其产量和环境反应差异很大；最近的研究表明，与不同微生物群的关联可能有助于品种产量的变化。利用普通园林试验研究了12个成熟柳枝稷栽培品种土壤微生物组的变化，并进一步研究了根系性状和土壤条件对土壤微生物组结构的影响。研究发现，不同品种的平均根径差异可达33%，而且不同品种的土壤微生物群也不同。品种对土壤细菌群落的影响大于真菌群落，但两者都受土壤性质的强烈影响。根系性状对微生物群落结构的影响较弱，而根系长度对真菌群落的变化有影响。与土壤群落不同，根系细菌群落不按品种分组，而是基于样本子集。不同生态型土壤微生物生物量碳氮丰度及优势菌门丰度存在差异，但品种间土壤微生物组差异大于生态型间差异。我们的研究结果表明，没有一种土壤微生物组适用于所有柳枝稷品种，甚至适用于每种生态型。根系性状、微生物生物量和某些土壤细菌丰度的这些细微但显著的差异可以解释品种产量和环境反应的差异。
Microbial communities help plants access nutrients and tolerate stress. Some microbiomes are specific to plant genotypes and, therefore, may contribute to intraspecific differences in plant growth and be a promising target for plant breeding. Switchgrass (Panicum virgatum) is a potential bioenergy crop with broad variation in yields and environmental responses; recent studies suggest that associations with distinct microbiomesmay contribute to variation in cultivar yields. We used a common garden experiment to investigate variation in 12 mature switchgrass cultivar soil microbiomes and, furthermore, to examine how root traits and soil conditions influence microbiome structure. We found that average root diameter varied up to 33% among cultivars and that the cultivars also associated with distinct soil microbiomes. Cultivar had a larger effect on the soil bacterial than fungal community but both were strongly influenced by soil properties. Root traits had a weaker effect on microbiome structure but root length contributed to variation in the fungal community. Unlike the soil communities, the root bacterial communities did not group by cultivar, based on a subset of samples. Microbial biomass carbon and nitrogen and the abundance of several dominant bacterial phyla varied between ecotypes but overall the differences in soil microbiomes were greater among cultivars than between ecotypes. Our findings show that there is not one soil microbiome that applies to all switchgrass cultivars, or even to each ecotype. These subtle but significant differences in root traits, microbial biomass, and the abundance of certain soil bacteria could explain differences in cultivar yields and environmental responses.