A Novel Histochemical Method for a Simultaneous Staining of Melanin and Collagen Fibers
A Novel Histochemical Method for a Simultaneous Staining of Melanin and Collagen Fibers
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DOI：
10.1369/0022155410398001
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发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
Campos, Antonio
中科院分区：
文献类型：
--
作者：
Carriel, Victor S.;Aneiros-Fernandez, Jose;Campos, Antonio
对于黑素细胞病变的组织病理学诊断，可能需要识别黑色素，因为其可视化是非特异性的苏木精-伊红（HE）。Fontana-Masson（FM）技术用于这种类型病变的组织病理学，它允许识别色素，但失去了所有的形态学参数。作者描述了FM方法的改进，用于同时评价细胞外基质的形态、黑色素的亲银反应和胶原纤维，为此他们开发了Fontana-Masson picrosirius（FMPS）方法。不同黑素细胞病变的活检用于HE、FM和FMPS方法的性能。用ImageJ软件确定黑色素、胶原蛋白和上皮细胞的反应的像素强度。FMPS方法允许评价形态特征，同时以高强度识别黑色素和胶原纤维。该方法在黑色素的鉴定方面与FM没有显著差异，保持了其灵敏度和特异性。此外，在形态学显示方面与HE无差异。然而，FMPS在识别胶原纤维方面具有明显的上级优势。FMPS方法结合了形态学和组织化学参数，可用于研究黑素细胞来源的色素性病变。（J Histochem Cytochem 59：270-277，2011）
For the histopathologic diagnosis of melanocytic lesions, it could be necessary to identify the melanin pigment because its visualization is unspecific with hematoxylin-eosin (HE). The Fontana-Masson (FM) technique is used in histopathology in this type of lesion, which allows the identification of the pigment, but it loses all the morphologic parameters. The authors describe a modification to the FM method, for the evaluation of the morphology, the argentaffin reaction of the melanin, and collagens fibers of the extracellular matrix simultaneously, for which they have developed the Fontana-Masson picrosirius (FMPS) method. Biopsies of different melanocytic lesions were used for the performance of the HE, FM, and FMPS methods. The pixel intensity of the reaction for melanin, collagen, and epithelium was determined with ImageJ software. The FMPS method allows the evaluation of morphological characteristics, identifying the melanin pigment and collagen fibers with high intensity simultaneously. This method does not differ significantly from FM in the identification of melanin, maintaining its sensitivity and specificity. In addition, it does not differ in the demonstration of the morphology with HE. However, FMPS is significantly superior in the identification of collagen fibers. The FMPS method combines morphological and histochemical parameters that could be useful in the study of pigmented lesions of melanocytic origin. (J Histochem Cytochem 59:270-277, 2011)