ADENOSINE-TRIPHOSPHATE CONSUMPTION BY BACTERIAL ARGINYL-TRANSFER RIBONUCLEIC-ACID SYNTHETASES
ADENOSINE-TRIPHOSPHATE CONSUMPTION BY BACTERIAL ARGINYL-TRANSFER RIBONUCLEIC-ACID SYNTHETASES
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DOI：
10.1042/bj1790407
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发表时间：
1979-01-01
影响因子：
4.1
通讯作者：
CHARLIER, J
中科院分区：
文献类型：
--
作者：
GODEAU, JM;CHARLIER, J
用萤火虫荧光素酶法研究了大肠杆菌和嗜热脂肪芽孢杆菌的乙酰-tRNA合成酶对ATP的消耗。精氨酰-tRNA合成酶。大肠杆菌仅利用ATP以1：1的化学计量进行tRNA的氨酰化。来自B的乙酰-tRNA合成酶的ATP活性。显示了在不存在tRNAArg的情况下嗜热脂肪菌的生长。Dowex色谱法显示ADP的形成由嗜热酶;在氨酰化条件下，AMP也形成了化学计量的量与乙酰基-tRNA的形成。
ATP consumption by arginyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus was investigated by the firefly luciferin-luciferase assay. Arginyl-tRNA synthetase from E. coli utilizes ATP only for aminocylation of tRNA with a 1:1 stoicheiometry. An ATP activity of arginyl-tRNA synthetase from B. stearothermophilus in the absence of tRNAArg was shown. Dowex chromatography revealed the formation of ADP by the thermophile enzyme; under aminoacylation conditions, AMP was also formed in amounts stoicheiometric with arginyl-tRNA formation.