Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa
Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa
复制标题
鉴定一种新型线粒体蛋白，短胚后根 1 (SPR1)，参与水稻根发育和铁稳态
DOI：
10.1111/j.1469-8137.2010.03513.x
复制
发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Mao, Chuanzao
中科院分区：
文献类型：
--
作者：
Jia, Liqiang;Wu, Zhongchang;Mao, Chuanzao
水稻突变体Oryza sativa short postembryonic roots 1 （Osspr1）已被鉴定。它具有短的胚后根，包括不定根和侧根，其叶片中的铁含量较低。OsSPR1通过图谱克隆得到鉴定。它编码了一种具有犰狳样重复结构域的新型线粒体蛋白。Osspr1突变体表现为根细胞伸长减少。与野生型相比，突变体的铁含量发生了显著变化。在枝条中也观察到类似的锰和锌浓度变化模式。突变体的互补证实了OsSPR1参与水稻胚后根伸长和铁稳态。OsSPR1被发现在整个植物的各种组织中普遍表达。通过策略I和II参与铁摄取和信号传导的各种基因的转录丰度在野生型和突变型植物的根中相似，但在突变型植物的叶片中更高。因此，一种新的线粒体蛋白参与根伸长并在金属离子稳态中发挥作用。
P>A rice mutant, Oryza sativa short postembryonic roots 1 (Osspr1), has been characterized. It has short postembryonic roots, including adventitious and lateral roots, and a lower iron content in its leaves.OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain.Osspr1 mutants exhibited decreased root cell elongation. The iron content of the mutant shoots was significantly altered compared with that of wild-type shoots. A similar pattern of alteration of manganese and zinc concentrations in shoots was also observed. Complementation of the mutant confirmed that OsSPR1 is involved in post-embryonic root elongation and iron homeostasis in rice. OsSPR1 was found to be ubiquitously expressed in various tissues throughout the plant. The transcript abundance of various genes involved in iron uptake and signaling via both strategies I and II was similar in roots of wild-type and mutant plants, but was higher in the leaves of mutant plants.Thus, a novel mitochondrial protein that is involved in root elongation and plays a role in metal ion homeostasis has been identified.