Oxalate induced expression of monocyte chemoattractant protein-1 (MCP-1) in HK-2 cells involves reactive oxygen species
Oxalate induced expression of monocyte chemoattractant protein-1 (MCP-1) in HK-2 cells involves reactive oxygen species
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DOI：
10.1007/s00240-005-0505-3
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发表时间：
2005-12-01
影响因子：
--
通讯作者：
Khan, SR
中科院分区：
文献类型：
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作者：
Habibzadegah-Tari, P;Byer, K;Khan, SR
草酸盐是代谢的有毒终产物，主要是因为其倾向于结晶并形成草酸钙，草酸钙在生理pH下不溶，并且经常沉积在非常不幸的部位，特别是肾脏。在目前的研究中，我们研究了琥珀酸诱导的损伤和单核细胞趋化蛋白-1（MCP-1）在HK-2细胞中的上调，HK-2细胞是一种来自正常人肾脏的近端肾小管上皮细胞系。将细胞暴露于草酸根离子不同的时间长度。测试培养基的LDH释放，LDH是细胞损伤标志物。从细胞中分离mRNA并进行逆转录酶-聚合酶链反应。结果表明，草酸盐暴露可导致细胞损伤，且具有时间和浓度依赖性。MCP-1的mRNA增加暴露于草酸盐和自由基清除剂，过氧化氢酶和超氧化物歧化酶处理后减少。这些数据支持活性氧在诱导肾上皮细胞表达MCP-1中的重要性。据我们所知，这是第一个报告MCP-1的表达和上调草酸暴露在HK-2细胞。
Oxalate is a toxic end product of metabolism largely because of its propensity to crystallize and form calcium oxalate, which is insoluble at physiologic pH and often deposits at very unfortunate sites, notably the kidneys. In the current study, we investigated the oxalate-induced injury and up-regulation of monocyte-chemoattractant protein-1 (MCP-1) in HK-2 cells, a proximal tubular epithelial cell line derived from normal human kidney. The cells were exposed to oxalate ions for different lengths of time. The culture media was tested for LDH release, a cell injury marker. mRNA was isolated from the cells and subjected to reverse transcriptase-polymerase chain reaction. The data showed that oxalate exposure resulted in cell injury in a time and concentration dependent manner. The MCP-1 mRNA increased following exposure to oxalate and was reduced upon treatment with free radical scavengers, catalase and superoxide dismutase. These data support the importance of reactive oxygen species in the induction of expression of MCP-1 in renal epithelial cells. To our knowledge, this is the first report of MCP-1 expression and its upregulation by oxalate exposure in HK-2 cells.