Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4
Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4
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DOI：
10.1006/bbrc.1996.0059
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发表时间：
1996-01-05
影响因子：
3.1
通讯作者：
Power, CA
中科院分区：
文献类型：
--
作者：
Hoogewerf, AJ;Black, D;Power, CA
我们克隆了人CC趋化因子受体-4（CC CKR-4）的鼠同源物。在用人和鼠CC CKR-4 cDNA转染的未分化HL-60细胞中进行的平衡竞争结合试验中，[I-125]巨噬细胞炎性蛋白-lot与人和鼠CC CKR-4结合的IC 50值分别为14.5 +/- 9.0 nM和10.1 +/- 3.0 nM，[（125）]RANTES与人和鼠CC CKR-4结合的IC 50值分别为9.3 +/- 3.0 nM和5.7 +/- 2.6 nM。鼠CC CKR-4的cDNA克隆是1531 bp，并且最大的开放阅读框编码与人CC CKR-4具有85%同一性的360个氨基酸的蛋白质。通过北方印迹分析在胸腺和T细胞系中检测到小鼠CC CKR-4。趋化因子与CC CKR-4直接结合的首次报道表明，高度同源的人和鼠受体具有相似的结合特征和组织分布。(C)出版社：Academic Press，Inc.
We have cloned the murine homologue of human CC Chemokine Receptor-4 (CC CKR-4). In equilibrium competition binding assays performed in undifferentiated HL-60 cells transfected with human and murine CC CKR-4 cDNA, the IC50 values for the binding of [I-125]macrophage inflammatory protein-lot to human and murine CC CKR-4 were 14.5 +/- 9.0 nM and 10.1 +/- 3.0 nM, respectively, and the IC50 values for the binding of [(125)]RANTES to human and murine CC CKR-4 were 9.3 +/- 3.0 nM and 5.7 +/- 2.6 nM, respectively. The cDNA clone for murine CC CKR-4 is 1531 bp, and the largest open reading frame encodes a protein of 360 amino acids that is 85% identical to human CC CKR-4. Murine CC CKR-4 was detected in the thymus and T-cell lines by Northern blot analysis. This first report of direct binding of chemokines to CC CKR-4 demonstrates that the highly homologous human and murine receptors have similar binding characteristics and tissue distribution. (C) 1996 Academic Press, Inc.