EB virus induction is associated with B-cell maturation.
EB virus induction is associated with B-cell maturation.
复制标题
EB 病毒诱导与 B 细胞成熟相关。
作者：
D. Crawford;I. Andó
采用间接免疫荧光法对携带EB病毒基因组的b细胞系进行了b细胞活化或成熟抗原和病毒抗原的双重染色。绝大多数细胞表达活化抗原，而浆细胞抗原PC1仅存在于少数细胞上。该抗原染色与EB病毒衣壳抗原染色加倍。因此，它标志着病毒复制周期中的细胞，这些细胞对EB病毒核抗原EBNA呈阴性。我们认为，在携带EB病毒基因组的b细胞系中，少数细胞成熟到表达浆细胞标记的阶段会导致EBNA表达的丧失，从而导致细胞的永生化状态的丧失。这将允许裂解周期的完成，产生病毒颗粒和细胞死亡。讨论了这些发现的意义。
EB virus genome-carrying B-cell lines have been double-stained for B-cell activation or maturation antigens and viral antigens using indirect immunofluorescence. The vast majority of cells express activation antigens, whereas the plasma cell antigen PC1 is present on only a few cells. Staining for this antigen doubles with staining for EB viral capsid antigen. Thus it marks cells in the virus replicative cycle, and these cells are negative for the EB virus nuclear antigen EBNA. We suggest that within EB virus genome-carrying B-cell lines the maturation of a few cells to the stage of expression of plasma-cell markers leads to the loss of EBNA expression and consequent loss of the immortalized state of the cell. This would allow completion of the lytic cycle with the production of virus particles and cell death. The implications of these findings are discussed.