Immunogenetics and rheumatoid arthritis.
Immunogenetics and rheumatoid arthritis.
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免疫遗传学和类风湿性关节炎。
DOI：
10.1016/0167-5699(88)91249-2
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
P. Dyer
中科院分区：
文献类型：
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作者：
D. Grennan;P. Dyer
由T细胞识别的DM Grennan和PA DyerW. Oilier（伦敦医院）报道在26%的dr4阳性RA受试者中存在DW14变异，但在dr4阳性对照中仅存在7%，而其他DW类型在相似的人群中也有发现。部分dr匹配的RA和对照受试者。先前使用DQo~和DQI3探针和一系列限制性内切酶进行的RFLP分析已经确定了在所有RA受试者中发现的片段1的“omega”模式，这些片段1分型为DW14。反过来，DW14和omega RFLP模式在B15 BfS DR4单倍型中都被发现。针对dqi3编码特异性的单克隆抗体TAIO的血清学分型也被用于细分dr4阳性人群。在正常的高加索人dr4阳性，dq3阳性（DQ位点的血清学分型显示三种变体，DQWl， 1和3）人群中，42%的个体为taio阳性。在RA中，TAIO阴性个体的比例增加，并且TAIO阴性与BW62 BfS DR4单倍型相关（J. Awad，伦敦医院）。然而，B44 BfS DR4， talo阳性单倍型尤其发生在严重形式的RA中。费尔蒂综合征是一种严重的类风湿疾病，参加会议的几个小组已经对其进行了研究。该综合征是一种罕见的类风湿性疾病并发症，临床上表现为均匀性，以脾肿大、白细胞减少和常伴有严重关节炎为特征。Felty的患者中约95%为dr4阳性，而没有这种关节外并发症的医院类风湿性关节炎患者中约60%为dr4阳性。a . So（皇家研究生医学院）对一株dr4阳性纯合细胞系的DQI3链进行了测序，并确定了13条链序列负责赋予TAIO反应性。该序列的知识随后用于构建等位基因特异性寡核苷酸探针，该探针用于来自RA， Felty和正常受试者的pvull消化DNA样本的Southern印迹。使用该探针，68%的Felty阳性，28%的dr4阳性
from DM Grennan and PA Dyer recognized by T cells. W. Oilier (London Hospital) reported the DW14 variant in 26% of DR4-positive RA subjects but in only 7% of DR4-positive controls, whereas the other DW types were seen in similar pro-. portions of DR-matched RA and control subjects. Previous RFLP analyses using DQo~ and DQI3 probes and a range of restriction endonucleases have defined an'omega'pattern of fragments 1 found in all RA subjects typing for DW14. In turn, both DW14 and the omega RFLP pattern are found particularly on the B15 BfS DR4 haplotype.Serological typing with a monoclonal antibody to the DQI3-encoded specificity, TAIO, has also been used to subdivide DR4-positive populations. In a normal Caucasian DR4-positive, DQ3-positive (serological typing at the DQ locus reveals three variants, DQWl, 1 and 3) population, 42% of individuals are TAIO-positive. The proportion of TAIO-negative individuals is increased in RA and TAIO negativity is associated with the BW62 BfS DR4 haplotype (J. Awad, London Hospital). However, the B44 BfS DR4, TAlO-positive haplotype occurred particularly in severe forms of RA. Felty's syndrome is one form of severe rheumatoid disease which has been studied by several groups attending the meeting. This syndrome is an uncommon complication of rheumatoid disease which appears clinically homogeneous and is characterized by splenomegaly, leucopenia and often severe arthritis. About 95% of Felty's patients are DR4-positive, compared with about 60% of hospital RA patients without this extra-articular complication. A. So (Royal Postgraduate Medical School) has sequenced the DQI3 chain of a DR4-positive homozygous cell line and determined the 13 chain sequence responsible for conferring TAIO reactivity. Knowledge of this sequence was subsequently used to construct an allele-specific oligonucleotide probe which was used in Southern blots of Pvull-digested DNA samples from RA, Felty's and normal subjects. Using this probe, 68% of Felty's, 28% of DR4-positive