The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes.
The yeast homolog to mouse Tcp-1 affects microtubule-mediated processes.
复制标题
小鼠 Tcp-1 的酵母同源物影响微管介导的过程。
DOI：
10.1128/mcb.11.5.2629-2640.1991
复制
发表时间：
1991
影响因子：
5.3
通讯作者：
Culbertson,MR
中科院分区：
文献类型：
--
作者：
Ursic,D;Culbertson,MR
黑腹果蝇（Drosophila melanogaster）和小鼠Tcp-1同源物，编码无尾复合多肽1（tailless complex polypeptide 1，TCP 1）。小鼠t复合体由于其对胚胎发生、精子分化和功能的影响，60年来一直受到研究。TCP 1是酵母细胞中的一个必需基因，位于染色体4 R上，与14号染色体相连。酵母、果蝇和小鼠细胞中TCP 1编码的蛋白质具有61%至72%的氨基酸序列相同性，表明TCP 1基因产物具有原始功能。为了评估功能，我们在酵母基因中构建了一个冷损伤隐性突变（tcpl-1）。携带tcp 1 - 1突变的细胞在限制性温度15°C下线性生长而不是指数生长，在基本培养基中的世代时间约为32 h。多核和无核细胞积累随着时间的推移，表明线性生长动力学可以解释为产生的无核芽不能进一步细胞分裂。此外，多核和无核细胞含有抗α-微管蛋白抗体检测到的形态学异常结构。这些异常出现的动力学表明，它们是TCP 1蛋白功能丧失的直接后果，而不是细胞死亡的延迟、间接后果。我们还观察到携带tcp 1 - 1的菌株对抗有丝分裂化合物高度敏感。总之，这些观察结果表明，TCP 1蛋白影响微管介导的过程。
ASaccharomyces cerevisiaehomolog toDrosophila melanogasterand mouseTcp-1encoding tailless complex polypeptide 1 (TCP1) has been identified, sequenced, and mapped. The mouse t complex has been under scrutiny for six decades because of its effects on embryogenesis and sperm differentiation and function.TCP1is an essential gene in yeast cells and is located on chromosome 4R, linked topet14.TheTCP1-encoded proteins in yeast,Drosophila,and mouse cells share between 61 and 72% amino acid sequence identities, suggesting a primordial function for theTCP1gene product. To assess function, we constructed a cold-impaired recessive mutation(tcpl-1)in the yeast gene. Cells carrying thetcp1-1mutation grew linearly rather than exponentially at the restrictive temperature of 15°C with a generation time of approximately 32 h in minimal medium. Both multinucleate and anucleate cells accumulated with time, suggesting that the linear growth kinetics may be explained by the generation of anucleate buds incapable of further cell division. In addition, the multinucleate and anucleate cells contained morphologically abnormal structures detected by anti-α-tubulin antibodies. The kinetics of appearance of these abnormalities suggest that they are a direct consequence of loss of function of the TCP1 protein and not a delayed, indirect consequence of cell death. We also observed that strains carryingtcp1-1were hypersensitive to antimitotic compounds. Taken together, these observations imply that the TCP1 protein affects microtubule-mediated processes.