Kinetics of oligonucleotide hybridization to photolithographically patterned DNA arrays
Kinetics of oligonucleotide hybridization to photolithographically patterned DNA arrays
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DOI：
10.1016/j.ab.2006.07.042
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发表时间：
2006-11-15
影响因子：
2.9
通讯作者：
Frank, Curts W.
中科院分区：
文献类型：
--
作者：
Glazer, Marc;Fidanza, Jacqueline A.;Frank, Curts W.
已经测量了寡核苷酸靶标与使用 Affyrnetrix 开发的光刻制造方法合成的寡核苷酸探针阵列的杂交动力学。通过监测荧光标记靶标与阵列的杂交，在室温和 45°C 下确定基本吸附参数 k(a)、k(d) 和 K 的值。这些参数的值和吸附的目标密度（
The hybridization kinetics of oligonucleotide targets to oligonucleotide probe arrays synthesized using photolithographic fabrication methods developed by Affyrnetrix have been measured. Values for the fundamental adsorption parameters, k(a), k(d), and K, were determined at both room temperature and 45 degrees C by monitoring the hybridization of fluorescently labeled targets to the array. The values for these parameters and the adsorbed target density (