Characterization of two novel protocadherins (PCDH8 and PCDH9) localized on human chromosome 13 and mouse chromosome 14
Characterization of two novel protocadherins (PCDH8 and PCDH9) localized on human chromosome 13 and mouse chromosome 14
复制标题
DOI：
10.1006/geno.1998.5467
复制
发表时间：
1998-10-01
期刊：
影响因子：
4.4
通讯作者：
Lalande, M
中科院分区：
文献类型：
--
作者：
Strehl, S;Glatt, K;Lalande, M
原钙粘蛋白是钙粘蛋白超家族的钙依赖性细胞-细胞粘附和识别蛋白的一个亚家族。在这项研究中，我们描述了两个新的原钙粘蛋白，PCDH 8和PCDH 9，构成了一个新的连锁群上的人类13号染色体和小鼠14号染色体的分离和表征。与其他原钙粘蛋白一样，这两种基因主要在大脑中表达，但PCDH 9也在更广泛的组织中表达，并且表达模式似乎受到发育调控。我们已经确定了PCDH 8的基因组组织，这与其他钙粘蛋白亚家族的基因组组织有显着不同。与通常由15-17个外显子组成并且在内含子位置上具有显著程度的保守性的经典钙粘蛋白和桥粒钙粘蛋白相比，PCDH 8仅由三个外显子组成并且在胞外结构域中缺乏内含子。第一个外显子编码胞外结构域、跨膜区和部分胞质尾区。第二个外显子编码胞质区域的剩余部分，并且部分未翻译。钙粘蛋白亚家族的基因组结构的差异将在钙粘蛋白超家族进化的背景下进行讨论。(C)北京：科学出版社.
The protocadherins are a subfamily of the calcium-dependent cell-cell adhesion and recognition proteins of the cadherin superfamily. In this study we describe the isolation and characterization of two novel protocadherins, PCDH8 and PCDH9, that constitute a new linkage group on human chromosome 13 and mouse chromosome 14. Like other protocadherins both genes are predominantly expressed in brain, but PCDH9 is also expressed in a broader variety of tissues, and the expression patterns appear to be developmentally regulated. We have determined the genomic organization of PCDH8, which differs significantly from that of the other cadherin subfamilies. In contrast to the classical and desmosomal cadherins, which in general consist of 15-17 exons and share a remarkable degree of conservation in intron position, PCDH8 consists of only three exons and lacks introns in the extracellular domain. The first exon encodes the extracellular domain, the transmembrane region, and part of the cytoplasmic tail. The second exon encodes the remainder of the cytoplasmic region and is partially untranslated. The differences in the genomic structure of cadherin subfamilies will be discussed in the context of the evolution of the cadherin superfamily. (C) 1998 Academic Press.