Proteomics Computational Analyses Suggest That the Envelope Glycoproteins of Segmented Jingmen Flavi-Like Viruses are Class II Viral Fusion Proteins (β-Penetrenes) with Mucin-Like Domains
Proteomics Computational Analyses Suggest That the Envelope Glycoproteins of Segmented Jingmen Flavi-Like Viruses are Class II Viral Fusion Proteins (β-Penetrenes) with Mucin-Like Domains
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DOI：
10.3390/v12030260
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发表时间：
2020-03-01
期刊：
影响因子：
4.7
通讯作者：
Garry, Robert E.
中科院分区：
文献类型：
--
作者：
Garry, Courtney E.;Garry, Robert E.
荆门病毒是一种新发现的节段性类黄病毒，广泛分布于蜱类中，与人类发热性疾病有关。计算分析表明，荆门类黄病毒糖蛋白具有II类病毒融合蛋白的结构特征，包括由β折叠和短α螺旋组成的胞外结构域、具有界面疏水性的融合肽和三结构域结构。荆门类黄病毒糖蛋白的氨基端富含丝氨酸、苏氨酸和脯氨酸，这是粘蛋白样结构域的特征。预计几种丝氨酸和苏氨酸可通过添加O-连接聚糖进行修饰。一些糖蛋白被预测具有位于跨膜锚之前的额外粘蛋白样结构域，而其他糖蛋白被预测具有由两个α-螺旋组成的茎。黄病毒囊膜蛋白和荆门黄病毒糖蛋白可能是从一个共同的II类前体糖蛋白分化而来的，在分化后获得了一个或多个粘蛋白样结构域。
Jingmen viruses are newly described segmented flavi-like viruses that have a worldwide distribution in ticks and have been associated with febrile illnesses in humans. Computational analyses were used to predict that Jingmen flavi-like virus glycoproteins have structural features of class II viral fusion proteins, including an ectodomain consisting of beta-sheets and short alpha-helices, a fusion peptide with interfacial hydrophobicity and a three-domain architecture. Jingmen flavi-like virus glycoproteins have a sequence enriched in serine, threonine, and proline at the amino terminus, which is a feature of mucin-like domains. Several of the serines and threonines are predicted be modified by the addition of O-linked glycans. Some of the glycoproteins are predicted to have an additional mucin-like domain located prior to the transmembrane anchor, whereas others are predicted to have a stem consisting of two alpha-helices. The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence.