Cis-Acting Transcriptional Repression Establishes a Sharp Boundary in Chordate Embryos
Cis-Acting Transcriptional Repression Establishes a Sharp Boundary in Chordate Embryos
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DOI：
10.1126/science.1222488
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发表时间：
2012-08-24
期刊：
影响因子：
56.9
通讯作者：
Satou, Yutaka
中科院分区：
文献类型：
--
作者：
Imai, Kaoru S.;Daido, Yutaka;Satou, Yutaka
骨形态发生蛋白（BMP）信号转导在动物胚胎背腹（DV）模式形成中的功能在双侧性动物中是保守的。在脊椎动物中，BMP配体反背化形态发生蛋白（Admp）在背侧表达并移动到相反侧以指定腹侧命运。在这里，我们表明，针头是一种拮抗剂特异性Admp的作用，在建立DV轴的干表皮的海鞘玻璃海鞘胚胎。针头和Admp串联存在于从节肢动物到脊椎动物的各种动物的基因组中。这种基因组构型对于这些基因的互斥表达是重要的，因为针头转录直接干扰Admp增强子的作用。我们的数据表明，这种双重负调控机制在动物中广泛保守。
The function of bone morphogenetic protein (BMP) signaling in dorsoventral (DV) patterning of animal embryos is conserved among Bilateria. In vertebrates, the BMP ligand antidorsalizing morphogenetic protein (Admp) is expressed dorsally and moves to the opposite side to specify the ventral fate. Here, we show that Pinhead is an antagonist specific for Admp with a role in establishing the DV axis of the trunk epidermis in embryos of the ascidian Ciona intestinalis. Pinhead and Admp exist in tandem in the genomes of various animals from arthropods to vertebrates. This genomic configuration is important for mutually exclusive expression of these genes, because Pinhead transcription directly disturbs the action of the Admp enhancer. Our data suggest that this dual negative regulatory mechanism is widely conserved in animals.