Analysis of Kinetic Intermediates in Single-Particle Dwell-Time Distributions
Analysis of Kinetic Intermediates in Single-Particle Dwell-Time Distributions
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DOI：
10.1016/j.bpj.2010.04.049
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发表时间：
2010-07-21
影响因子：
3.4
通讯作者：
van Oijen, Antoine M.
中科院分区：
文献类型：
--
作者：
Floyd, Daniel L.;Harrison, Stephen C.;van Oijen, Antoine M.
许多生物和化学过程都要经过一个或多个中间步骤。单分子轨迹的停留时间分布的统计分析使研究不能直接观察到的中间步骤成为可能。在这里，我们讨论了随机性参数和模型拟合在确定随机过程的步数中的应用。通过模拟实例，我们展示了这些技术的一些局限性。我们讨论了散粒噪声和各个步长转换率之间的异质性如何影响步数的准确确定。最后，我们探讨了多步反应中的动力学无序现象，并表明该现象可以掩盖限速中间步骤的存在。
Many biological and chemical processes proceed through one or more intermediate steps. Statistical analysis of dwell-time distributions from single molecule trajectories enables the study of intermediate steps that are not directly observable. Here, we discuss the application of the randomness parameter and model fitting in determining the number of steps in a stochastic process. Through simulated examples, we show some of the limitations of these techniques. We discuss how shot noise and heterogeneity among the transition rates of individual steps affect how accurately the number of steps can be determined. Finally, we explore dynamic disorder in multistep reactions and show that the phenomenon can obscure the presence of rate-limiting intermediate steps.