Six genes of ompA family shuffling for development of polyvalent vaccines against Vibrio alginolyticus and Edwardsiella tarda
Six genes of ompA family shuffling for development of polyvalent vaccines against Vibrio alginolyticus and Edwardsiella tarda
复制标题
ompA家族的六个基因改组用于开发抗溶藻弧菌和迟缓爱德华氏菌多价疫苗
DOI：
10.1016/j.fsi.2018.02.022
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发表时间：
2018-04-01
影响因子：
4.7
通讯作者：
Li, Hui
中科院分区：
文献类型：
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作者：
Cheng, Zhi-xue;Chu, Xiao;Li, Hui
由于水产养殖生态系统的复杂性，针对一种以上病原体的多价疫苗尤为重要。我们先前已经表明，通过用副溶血性弧菌VP 0764引物改组来自不同细菌的六个ompA基因来开发多价疫苗。在此，我们使用相同的6个基因，溶藻弧菌VA 0764和VA 1186，副溶血弧菌VP 0764和VP 1186，大肠杆菌E. tarda ompA和E. coliompA，而E. tarda ompA引物开发新的多价疫苗。通过这种方法，我们鉴定了7种潜在的多价疫苗，它们对溶藻弧菌和大肠杆菌都有效。迟发性感染此外，还在三个组中探索了由疫苗触发的先天免疫，即无保护（组I）、针对溶藻弧菌的保护（组II）以及针对溶藻弧菌和E.迟发性痴呆（Ⅲ组）。IL-1 β、IL-6、IL-8、C3 b和NF-kB的转录在II组和III组中显著增加，而在I组中无显著性差异，其中III组的表达水平高于II组。此外，琥珀酸脱氢酶活性在三组之间存在差异。这些结果表明，扩大了具有相同改组基因但不同引物的多价疫苗库，并促进了对基于疫苗诱导天然免疫的多价疫苗机制的理解。
Polyvalent vaccines against more than one species of pathogens are especially important due to the complex ecosystem in aquaculture. We have previously shown that the development of polyvalent vaccines by shuffling six ompA genes from different bacteria with V. parahaemolyticus VP0764 primers. Here, we used the same 6 genes, V. alginolyticus VA0764 and VA1186, V. parahaemolyticus VP0764 and VP1186, E. tarda ompA and E. coli ompA, but with E. tarda ompA primers to develop new polyvalent vaccines. By this approach, we identified 7 potential polyvalent vaccines that were effective against both V. alginolyticus and E. tarda infections. Furthermore, the innate immunity triggered by the vaccines were also explored in three groups, no protection (group I), protection against V. alginolyticus (group II), and protection against both V. alginolyticus and E. tarda (group III). The transcription of IL-1 beta, IL-6, IL-8, C3b and NF-kB were significantly increased in group II and group III but not group I, where the expression level of group III was higher than group II. In addition, differential activities of succinate dehydrogenase were detected among the three groups. These results indicate the expansion of polyvalent vaccine reservoir with the same shuffling genes but different primers, and promote the understanding of the mechanisms of polyvalent vaccines based on vaccine-induced innate immunity.