The development-specific ssp1 and ssp2 genes of Sclerotinia sclerotiorum encode lectins with distinct yet compensatory regulation
The development-specific ssp1 and ssp2 genes of Sclerotinia sclerotiorum encode lectins with distinct yet compensatory regulation
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DOI：
10.1016/j.fgb.2010.03.008
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
Rollins, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Li, Moyi;Rollins, Jeffrey A.
Ssp 1发育特异蛋白是核盘菌菌核和菌核中含量最丰富的可溶性蛋白。虽然与这些发育阶段密切相关，但Ssp1蛋白及其副产物Ssp2的功能尚不清楚。在这项研究中，蛋白质结构预测分析表明，Ssp1和Ssp2在结构上类似于岩藻糖特异性凝集素。为了了解这些丰富的发育特异性蛋白质的功能，产生了一种同核ssp 1缺失突变体。由此产生的突变体（三角洲ssp 1）显示野生型的生长和发展表型在文化中，但产生约50%的菌核补充潮霉素的文化。在潮霉素存在下，与野生型ssp 1基因互补可恢复正常的菌核形成。这表明Ssp1可能在对环境中遇到的含糖苷抗生素的抗性中发挥作用。虽然观察到果期萌发略有延迟，但没有发现ssp 1功能丧失对apothecial形态或生殖力的额外影响。当在Delta ssp 1突变体中检查ssp 2的表达时，发现其在菌核发育中较早表达，并且其编码的蛋白质在菌核和菌核中积累到较高水平。这些研究结果表明，调节补偿损失的Ssp1加上潜在的功能冗余凝集素积累在菌核和apotherapy。(C)2010年爱思唯尔公司All rights reserved.
The Ssp1 development-specific protein is the most abundant soluble protein in sclerotia and apothecia of Sclerotinia sclerotiorum. Although closely associated with these developmental stages, the functions of the Ssp1 protein and its paralog, Ssp2, are not known. In this study, protein structure prediction analysis revealed that Ssp1 and Ssp2 are structurally similar to fucose-specific lectins. In an effort to understand the function of these abundant, development-specific proteins, a homokaryotic ssp1 deletion mutant was generated. The resulting mutant (Delta ssp1) displays a wild-type growth and development phenotype in culture but produces approximately 50% fewer sclerotia in cultures supplemented with hygromycin. Genetic complementation with a wild-type copy of ssp1 restores normal sclerotium formation in the presence of hygromycin. This suggests that Ssp1 might play a role in resistance to glycoside-containing antibiotics encountered in the environment. Although a slight delay in carpogenic germination was observed, no additional effects of ssp1 loss-of-function were found in regards to apothecial morphology or fecundity. When the expression of ssp2 was examined in the Delta ssp1 mutant, it was found to be expressed earlier in sclerotial development and its encoded protein accumulated to higher levels in both sclerotia and apothecia. These findings suggest regulatory compensation for loss of Ssp1 coupled with potential functional redundancy among lectins accumulating in sclerotia and apothecia. (C) 2010 Elsevier Inc. All rights reserved.