Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana
Simultaneous suppression of three genes related to brassinosteroid (BR) biosynthesis altered campesterol and BR contents, and led to a dwarf phenotype in Arabidopsis thaliana
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DOI：
10.1007/s00299-010-0830-z
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发表时间：
2010-04-01
影响因子：
6.2
通讯作者：
Chung, In Sik
中科院分区：
文献类型：
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作者：
Chung, Ho Yong;Fujioka, Shozo;Chung, In Sik
我们利用RNA干扰构建体构建拟南芥转基因系，表达发夹双链RNA，用于DET2:DWF4:SMT2诱导序列特异性RNA沉默。在转基因植株中，与野生型植株相比，DET2、DWF4和SMT2的表达同时降低，油菜甾醇含量增加了420%。DET2、DWF4和SMT2酶的三重敲除也导致油菜素内酯（BR）特异性生物合成中间体的减少。含有RNA干扰结构的转基因植物由于发育改变而表现出半矮化表型。我们的研究结果表明，通过同时抑制参与BR生物合成的多个基因，植物结构的重新设计是可能的。
We generated transgenic lines of Arabidopsis thaliana with an RNA interference construct that expressed hairpin double-stranded RNA for DET2:DWF4:SMT2 to induce sequence-specific RNA silencing. In transgenic plants, expressions of DET2, DWF4, and SMT2 were simultaneously reduced, and the campesterol content was increased by up to 420% compared to the level in the wild-type plant. Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates. Transgenic plants harboring the RNA interference construct displayed a semi-dwarf phenotype due to altered development. Our findings indicate that redesigning of plant architecture is possible through simultaneous suppression of multiple genes involved in BR biosynthesis.