Application of preparative high-speed countercurrent chromatography for separation of elatine from Delphinium shawurense
Application of preparative high-speed countercurrent chromatography for separation of elatine from Delphinium shawurense
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DOI：
10.1080/10826070802424956
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发表时间：
2008-01-01
影响因子：
1.3
通讯作者：
Ito, Yoichiro
中科院分区：
文献类型：
--
作者：
Yang, Yi;Gu, Dongyu;Ito, Yoichiro
采用高速逆流色谱法首次实现了对沙雾翠雀花中依拉汀的分离。以乙酸乙酯-氯仿-甲醇-水（3：0.1：2：3，v/v）为溶剂体系，下层有机相为移动的相，转速为800 rpm。这产生了72 mg纯度超过97%的依拉汀，回收率约为95%。其化学结构经MS和NMR确证。
Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800rpm. This yielded 72mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR.