Stability of cytochromes c′ from psychrophilic and piezophilic Shewanella species: implications for complex multiple adaptation to low temperature and high hydrostatic pressure
Stability of cytochromes c′ from psychrophilic and piezophilic Shewanella species: implications for complex multiple adaptation to low temperature and high hydrostatic pressure
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嗜冷和嗜压希瓦氏菌物种细胞色素 c 的稳定性：对低温和高静水压的复杂多重适应的影响
DOI：
10.1007/s00792-019-01077-9
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发表时间：
2019
期刊：
影响因子：
2.9
通讯作者：
Sambongi Yoshihiro
中科院分区：
文献类型：
--
作者：
Suka Asako;Oki Hiroya;Kato Yuki;Kawahara Kazuki;Ohkubo Tadayasu;Maruno Takahiro;Kobayashi Yuji;Fujii Sotaro;Wakai Satoshi;Lisdiana Lisa;Sambongi Yoshihiro
嗜热菌二聚体细胞色素C ′的稳定性与嗜温菌同源物相比，主要是由于血红素周围和亚基-亚基界面的相互作用增强，这两个区域都是分子内部区域。在这里，我们发现，在同源细胞色素c ′的等效内部区域的相互作用，从两个嗜冷希瓦氏菌，底栖希瓦氏菌和希瓦氏菌violacea（SBCP和SVCP，分别）类似地削弱了相应的嗜冷希瓦氏菌livingstonensis和mesophilicShewanellamazonensis（SLCP和SACP，分别），和一致的稳定性SVCP，SLCP和SACP增加，在这个顺序。因此，来自嗜冷菌、嗜温菌和嗜热菌的细胞色素c ′的稳定性在其分子内部区域受到系统调节。然而，出乎意料的是，SBCP的稳定性显著高于SVCP，并且前者具有额外的分子表面相互作用。总的来说，SBCP和SVCP一样，内部相互作用减弱，但前者稳定在分子表面，这意味着复杂的多重适应的蛋白质，因为SBCP和SVCP的嗜冷来源也是嗜压的，在深海极端的低温和高静水压力环境中蓬勃发展。
The stability of dimeric cytochromec′ from a thermophile, as compared with that of a homologous mesophilic counterpart, is attributed to strengthened interactions around the heme and at the subunit–subunit interface, both of which are molecular interior regions. Here, we showed that interactions in the equivalent interior regions of homologous cytochromesc′ from two psychrophiles,Shewanella benthicaandShewanella violacea(SBCP and SVCP, respectively) were similarly weakened as compared with those of the counterparts of psychrophilicShewanella livingstonensisand mesophilicShewanella amazonensis(SLCP and SACP, respectively), and consistently the stability of SVCP, SLCP, and SACP increased in that order. Therefore, the stability of cytochromesc′ from the psychrophile, mesophile, and thermophile is systematically regulated in their molecular interior regions. Unexpectedly, however, the stability of SBCP was significantly higher than that of SVCP, and the former had additional molecular surface interactions. Collectively, SBCP had weakened interior interactions like SVCP did, but the former was stabilized at the molecular surface as compared with the latter, implying complex multiple adaptation of the proteins because the psychrophilic sources of SBCP and SVCP are also piezophilic, thriving in deep-sea extreme environments of low temperature and high hydrostatic pressure.