Impairment of angiogenic activity in the serum from patients with coronary aneurysms due to Kawasaki disease
Impairment of angiogenic activity in the serum from patients with coronary aneurysms due to Kawasaki disease
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DOI：
10.1253/circj.71.1052
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发表时间：
2007-07-01
影响因子：
3.3
通讯作者：
Kohno, Yoichi
中科院分区：
文献类型：
--
作者：
Higashi, Kouji;Terai, Masaru;Kohno, Yoichi
背景炎症介质在川崎病（KD）冠状动脉炎的发生发展中起重要作用，但KD血清对血管内皮细胞的影响尚不清楚。我们假设，血清活性刺激体外人脐静脉内皮细胞（HUVEC）管的形成可能会受损KD。方法和结果冠状动脉瘤患者的血清刺激HUVEC管的形成比血清没有冠状动脉瘤或发热的控制。在冠状动脉瘤患者中，KD治疗前血清血管生成活性已经降低（与健康对照组相比，p=0.03，与发热对照组相比，p=0.08），静脉注射免疫球蛋白加阿司匹林后血清血管生成活性增强（与健康对照组相比，p < 0.001，与发热对照组相比，p=0.002）;两种药物均不影响所研究的测定。与发展为小至中度动脉瘤的患者相比，后来发展为> 8 mm的巨大动脉瘤的患者的减少幅度更大（p=0.01）。血清血管生成活性的降低部分是由于血清刺激HUVEC增殖的活性降低所致。结论KD患者血清刺激HUVEC管形成的活性受损，这些患者后来发展为较大的冠状动脉瘤，这可能与血管损伤的严重程度有关。
Background The inflammatory mediators play an important role in the progression of coronary vasculitis in Kawasaki disease (KD), but effects of KD serum including inflammatory mediators on endothelial cells remain unknown. We hypothesized that serum activity to stimulate in vitro human umbilical vein endothelial cells (HUVEC) tube formation might be impaired in KD.Methods and Results Serum from patients with coronary aneurysms was less active in stimulating HUVEC tube formation than serum from patients without coronary aneurysms or febrile controls. In patients with coronary aneurysms, the reduction in the serum angiogenic activity was documented already before KD treatment (p=0.03 vs healthy controls, p=0.08 vs febrile controls) and enhanced after intravenous immune globulin plus aspirin (p < 0.001 vs healthy controls, p=0.002 vs febrile controls); both drugs did not affect the assay studied. This reduction was greater in patients who later developed giant aneurysms > 8 mm compared with those who developed small to moderate aneurysms (p=0.01). The reduced serum angiogenic activity was partly caused by the reduction in the serum activity of stimulating HUVEC proliferation.Conclusions Serum activity to stimulate HUVEC tube formation was impaired in KD patients who later developed larger coronary aneurysms, which may be associated with the severity of vascular injury.