Role played by Th2 type cytokines in IgE mediated allergy and asthma.
Role played by Th2 type cytokines in IgE mediated allergy and asthma.
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DOI：
10.4103/0970-2113.63609
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Niphadkar PV
中科院分区：
文献类型：
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作者：
Deo SS;Mistry KJ;Kakade AM;Niphadkar PV
最近的证据表明，变应原2型辅助T细胞(Th2)在产生IgE抗体的B细胞、肥大细胞和嗜酸性粒细胞的激活/募集中起着触发作用。早期生命中微生物暴露的减少是免疫系统中Th1/Th2平衡向前过敏Th2反应转变的原因。Th1细胞主要产生干扰素γ和迟发型超敏反应，Th2细胞分泌IL-4、IL-5、IL-6、IL-13，调节B细胞和嗜酸性粒细胞介导的反应。为了评估免疫系统的调节变化，我们研究了过敏和哮喘患者血清中细胞因子的变化，并与正常健康对照组进行了比较。应用免疫技术试剂盒，对170例各种过敏和哮喘患者进行了血清细胞因子的检测，以确定过敏和哮喘的触发因素或主要影响因素。我们的研究显示，IL-4、IL-5和IL-6水平在所有组均升高，但均无统计学意义。而IL-10、IL-13和肿瘤坏死因子α水平差异有高度统计学意义。此外，我们还发现了GM-CSF和IL-10的相关性。与不同的细胞因子有显著的相关性。这些患者大多表现为IgE水平升高。这项研究更好地了解了细胞因子是如何调节Th1和Th2免疫反应的平衡，以及细胞因子如何控制IgE的合成。进一步的研究将最终导致在临床治疗中改进IgE介导的变态反应的治疗策略。
Recent evidence suggest that allergen type 2 helper T cells (Th2) play a triggering role in the activation/recruitment of IgE antibody producing B cells, mast cells and eosinophils. Reduced microbial exposure in early life is responsible for a shift of Th1/Th2 balance in the immune system towards the pre-allergic Th2 response. The Th1 predominantly produce IFNγ and delayed type hypersensitivity while Th2 secrete IL-4, IL-5, IL-6, IL-13 and regulate B cell and eosinophil mediated responses. To assess regulatory changes in the immune system, in patients with allergy and asthma, we studied the cytokine profile in serum in comparison with normal healthy controls. A total of 170 patients with various allergies and asthmatic conditions were studied, for cytokines in the serum by ELISA using kits from Immunotech, and analyzed to identify the triggering factors or main contributors towards allergy and asthma. Our study showed increase in the levels of IL-4, IL-5 and IL-6 in all groups which were non- significant. But the levels of IL-10, IL-13 and TNF α were highly significant. Besides, we found correlation of GM-CSF with IL-10. Significant correlation with different cytokines was observed. Most of these patients showed increase in IgE levels. This study gives a better understanding of how cytokines are the mediators of balance of Th1 and Th2 immune responses and IgE synthesis is controlled by cytokines. Further studies will eventually lead to improved treatment strategies in the clinical management of IgE mediated allergy.