Multicolor super-resolution DNA imaging for genetic analysis.
Multicolor super-resolution DNA imaging for genetic analysis.
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多色超分辨率DNA成像用于遗传分析。
DOI：
10.1021/nl302069q
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发表时间：
2012-07-11
期刊：
影响因子：
10.8
通讯作者：
Selvin PR
中科院分区：
文献类型：
--
作者：
Baday M;Cravens A;Hastie A;Kim H;Kudeki DE;Kwok PY;Xiao M;Selvin PR
许多类型的癌症和神经退行性疾病是由基因组的异常和变异引起的。我们设计了一种高通量、低成本的高分辨率成像技术，用于检测遗传病相关基因的结构变异。我们最初将Nb.BbvCI内切核酸酶的所有七个切口位点定位在λ DNA上。然后，我们解决了密集标记模式的107个切口位点的人类BAC DNA被消化的Nb.BsmI和Nb.BbvCI内切酶。这种高密度导致几种染料比衍射极限更接近。总体而言，实现了100bp分辨率的详细DNA切口位点定位，这有可能揭示遗传变异的信息，并促进几种遗传疾病的医学诊断。
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
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影响因子：
9.8
作者：
Church DM;Goodstadt L;Hillier LW;Zody MC;Goldstein S;She X;Bult CJ;Agarwala R;Cherry JL;DiCuccio M;Hlavina W;Kapustin Y;Meric P;Maglott D;Birtle Z;Marques AC;Graves T;Zhou S;Teague B;Potamousis K;Churas C;Place M;Herschleb J;Runnheim R;Forrest D;Amos-Landgraf J;Schwartz DC;Cheng Z;Lindblad-Toh K;Eichler EE;Ponting CP;Mouse Genome Sequencing Consortium
通讯作者：
Mouse Genome Sequencing Consortium
影响因子：
3.1
作者：
van de Linde, Sebastian;Krstic, Ivan;Sauer, Markus
通讯作者：
Sauer, Markus
影响因子：
3.4
作者：
Aitken, Colin Echeverria;Marshall, R. Andrew;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
影响因子：
56.9
作者：
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
DOI：
10.1073/pnas.0401638101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Gordon, MP;Ha, T;Selvin, PR
通讯作者：
Selvin, PR