Hypothalamic estrogen receptor-immunoreactivity in prepubertal vs adult female guinea pigs.
Hypothalamic estrogen receptor-immunoreactivity in prepubertal vs adult female guinea pigs.
复制标题
青春期前与成年雌性豚鼠的下丘脑雌激素受体免疫反应性。
DOI：
10.1111/j.1365-2826.1994.tb00627.x
复制
发表时间：
1994
影响因子：
3.2
通讯作者：
Olster,DH
中科院分区：
文献类型：
--
作者：
Olster,DH
幼年豚鼠(18-20日龄)很少表现出前凸，因为雌二醇和黄体酮治疗会引起成年雌性的性行为。在体外放射配基结合分析中，青春期前和成年豚鼠的下丘脑和视前区中雌激素受体的浓度相似。本研究的目的是比较未成熟和成年豚鼠前脑不同区域的雌激素受体免疫反应性，以确定下丘脑和视前区中雌激素受体水平的年龄差异是否存在。取幼年(17天)和成年雌性(>；6周)大鼠前脑组织，切除卵巢6天后，用雅培公司的H_222抗人雌激素受体抗体进行雌激素受体免疫反应。青少年在所有与成人相同的区域都有雌激素受体免疫反应阳性细胞：视前内侧区、视前内侧核、终纹床核、脑室周围核、室旁核、背内侧核和弓状核、下丘脑腹外侧区和前部以及杏仁核。在雌激素受体免疫反应阳性的区域(视前内侧区、视前内侧核、脑室周围前核、弓状核和下丘脑腹外侧部)中，雌激素受体免疫阳性染色的唯一区域是下丘脑腹外侧的吻侧部分。青少年女性的雌激素受体免疫反应阳性细胞数平均比成年女性少30%(440±25vs626±25，P=0.001)。这些数据与下丘脑头端腹外侧区雌激素受体不足的假说一致，这可能是幼年雌性豚鼠对雌二醇反应明显不足的部分原因。
Juvenile guinea pigs (18–20 days old) rarely display lordosis in response to estradiol and progesterone treatments that elicit sexual behavior in adult females. Nor do immature animals release a preovulatory‐like surge of luteinizing hormone in response to estradiol.In vitroradioligand binding assays have revealed similar concentrations of estrogen receptors in thehypothalamus and preoptic area of prepubertal and adult guinea pigs. The aim of the present study was to compare estrogen receptor‐immunoreactivity in a variety of forebrain regions of immature and adult guinea pigs, to determine whether age differences in estrogen receptor levels inmore discrete portions of the hypothalamus and preoptic area exist. Forebrain tissue from juvenile (17 days) and adult females (> 6 weeks), ovariectomized 6 days previously, was processed forestrogen receptor‐immunoreactivity, using Abbott Laboratories' H222 anti‐human estrogen receptor antibody. Juveniles had estrogen receptor‐immunoreactive cells in all of the same regions as adults: medial preoptic area, medial preoptic nucleus, bed nucleus of the stria terminalis, periventricular, paraventricular, dorsomedial and arcuate nuclei, ventrolateral and anterior hypothalamic regions, and amygdala. Among the areas in which estrogen receptor‐immunoreactivity was quantified (medial preoptic area, medial preoptic nucleus, anterior periventricular nucleus, arcuate nucleus and ventrolateral hypothalamus), the only region in which an age difference in estrogen receptor‐immunostaining was observed was the rostral portion of the ventrolateral hypothalamus. Juvenile females had, on average, 30% fewer estrogen receptor‐immunoreactive cells in asample of this region than adults (440 ± 25 vs 626 ± 25, P = 0.001). These data are consistent with the hypothesis that insufficient estrogen receptors in the rostral ventrolateral hypothalamus may underlie, in part, the markedly deficient responses of juvenile female guinea pigs to estradiol.