IODINATION OF PROTEINS, GLYCOPROTEINS, AND PEPTIDES USING A SOLID-PHASE OXIDIZING-AGENT, 1,3,4,6-TETRACHLORO-3-ALPHA 6-ALPHA-DIPHENYL GLYCOLURIL (LODOGEN)
IODINATION OF PROTEINS, GLYCOPROTEINS, AND PEPTIDES USING A SOLID-PHASE OXIDIZING-AGENT, 1,3,4,6-TETRACHLORO-3-ALPHA 6-ALPHA-DIPHENYL GLYCOLURIL (LODOGEN)
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DOI：
10.1016/0003-2697(81)90703-x
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
LOWRY, PJ
中科院分区：
文献类型：
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作者：
SALACINSKI, PRP;MCLEAN, C;LOWRY, PJ
将一种新型市售氧化剂 1,3,4,6-四氯-3.α.,6.α.-二苯基甘脲（Iodogen）与氯胺-T 和固相乳过氧化物酶在蛋白质、糖蛋白和肽 [人、大鼠和猪激素] 的放射性碘标记中进行比较。描述了一种进行低水平碘化的方法，并用于确定最大 125I 掺入量。碘化蛋白质通过分析凝胶过滤柱纯化，肽通过反相高效液相色谱纯化。两种方法均旨在分析示踪剂以发现碘化引起的聚集体和分解产物的存在。所有制备的示踪剂均在各自的放射免疫测定中进行抗体稀释和剂量反应曲线测试。碘可用于多种蛋白质和肽，理论上可以在氧化损伤最小的情况下掺入 I，并且可以产生长达 3 个月稳定的示踪剂。
A new, commercially available oxidizing agent, 1,3,4,6-tetrachloro-3.alpha.,6.alpha.-diphenyl glycoluril (Iodogen), was compared with chloramine-T and solid-phase lactoperoxidase in the radioiodination of proteins, glycoproteins and peptides [human, rat and porcine hormones]. A method for performing low-level iodinations is described and was used to determine maximum 125I incorporation. Iodinated proteins were purified on analytical gel filtration columns and peptides by reverse-phase high-performance liquid chromatography. Both methods were designed to analyze the tracers for the presence of aggregate and breakdown products caused by the iodination. All tracers prepared were tested in antibody dilution and dose-response curves in their respective radioimmunoassays. Iodogen can be used for a wide range of proteins and peptides, can permit theoretical I incorporation with minimal oxidation damage and can produce tracer stable for up to 3 mo.