A computer interfaced dual-beam rapid scan stopped-flow system for the study for transients in enzyme reactions.
A computer interfaced dual-beam rapid scan stopped-flow system for the study for transients in enzyme reactions.
复制标题
用于研究酶反应瞬态的计算机接口双光束快速扫描停流系统。
DOI：
10.1016/0003-2697(75)90576-x
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发表时间：
1975
影响因子：
2.9
通讯作者：
J. L. Dye
中科院分区：
文献类型：
--
作者：
C. Suelter;R. B. Coolen;N. Papadakis;J. L. Dye
现有的快速扫描停止流动装置进行了改进，并与pdp - 8i计算机接口，以允许研究底物，瞬态中间体和酶催化反应产物的光谱变化。数据采集软件产生显著的实时信噪比增强，并大大降低了数据存储要求。采用300 ~ 540 nm扫描观察了马肝醇脱氢酶催化对亚硝基-n、n -二甲基苯胺与NADH的反应。对单一反应混合物的观察可以证实以前的报告[Dunn和Bernhard， Biochemistry10, 4569 (1971)；Schack和Dunn，《美联储第八次会议摘要》，欧洲。物化学。Soc。#303(1972)]的两个中间体，并建议在这个过程中还有第三个。
An existing rapid scan stopped-flow apparatus was modified and interfaced to a PDP-8 I Computer to permit study of changes in the spectra of substrates, transient intermediates, and products of enzyme-catalyzed reactions. The data-acquisition software produces significant real time signal-to-noise enhancement and greatly reduced data storage requirements. The reaction of p-nitroso-N,N-dimethyl aniline and NADH catalyzed by horse liver alcohol dehydrogenase was observed by scanning from 300 to 540 nm. It was possible from observation of a single reaction mixture to confirm previous reports [Dunn and Bernhard, Biochemistry10, 4569 (1971); Schack and Dunn, Abstracts 8th Meeting, Fed. Eur. Biochem. Soc. #303 (1972)] of two intermediates and to suggest yet a third in the process.