The physical action of cellulases revealed by a quartz crystal microbalance study using ultrathin cellulose films and pure
The physical action of cellulases revealed by a quartz crystal microbalance study using ultrathin cellulose films and pure
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DOI：
10.1021/bm700980b
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发表时间：
2008-01-01
影响因子：
6.2
通讯作者：
Wagberg, Lars
中科院分区：
文献类型：
--
作者：
Josefsson, Peter;Henriksson, Gunnar;Wagberg, Lars
使用高分辨率石英晶体微量平衡（QCM）对对模型纤维素膜的影响对模型纤维素膜的作用敏感，对纳米厚模型纤维素膜的微小变化敏感。发现内糖酸不仅产生新的末端组，而且会引起纤维素膜的肿胀。核植物酶迅速降解了膜，这被发现是QCM晶体上剩余的纤维素量的迅速减少。然而，薄膜的粘弹性变化也表明在降解过程中膜的软化。所选纤维素酶的定义混合物比仅仅是纤维菌水溶解酶引起的降解速率明显更高。讨论了纤维素酶的协同作用，并增加了内og葡萄糖酶肿胀效应和膜软化。
The effects of fungal cellulases on model cellulose films were studied using a high-resolution quartz crystal microbalance (QCM) sensitive to minute changes of the nanometer thick model cellulose films. It was found that endoglucanases not only produce new end groups but also cause a swelling of the cellulose film. The cellobiohydrolases degraded the films quickly, which was detected as a rapid decrease in the remaining amount of cellulose on the QCM crystal. However, changing viscoelastic properties of the films also indicated a softening of the film during the degradation. A defined mixture of selected cellulases caused a significantly higher rate of degradation than only cellobiohydrolases. Cellulase synergism is discussed with the endoglucanase swelling effects and film softening added.