The sigma E subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments
The sigma E subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments
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枯草芽孢杆菌 RNA 聚合酶的 sigma E 亚基存在于前孢子和母细胞区室中
DOI：
10.1128/jb.171.4.2216-2218.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
W. Haldenwang
中科院分区：
文献类型：
--
作者：
H. C. Carlson;W. Haldenwang
将孢子形成开始后3.5小时（t3.5）收获的枯草芽孢杆菌细胞分级分离成富含母细胞或前孢子组分的提取物，并对sigma E及其前体蛋白P31进行免疫学分析。我们通过Western印迹（免疫印迹）分析确定，在母细胞和前孢子提取物中存在等量的P31和sigma E。这一结果意味着，虽然sigma E直到发育阶段才合成，此时细胞被划分为前孢子和母细胞室，但它可能指导在这两种结构中表达的基因的转录。
Bacillus subtilis cells harvested 3.5 h after the onset of sporulation (t3.5) were fractionated into extracts enriched in either mother cell or forespore components and were analyzed immunologically for sigma E and its precursor protein, P31. We determined by Western blot (immunoblot) analysis that equivalent amounts of P31 and sigma E were present in both mother cell and forespore extracts. This result implies that, although sigma E is not synthesized until a stage in development when the cell is partitioned into progenitor forespore and mother cell compartments, it probably directs the transcription of genes that are expressed in both of these structures.