Several approaches for vitamin D determination by surface plasmon resonance and electrochemical affinity biosensors
Several approaches for vitamin D determination by surface plasmon resonance and electrochemical affinity biosensors
复制标题
DOI：
10.1016/j.bios.2012.07.077
复制
发表时间：
2013-02-15
影响因子：
12.6
通讯作者：
Mazzei, Franco
中科院分区：
文献类型：
--
作者：
Carlucci, Luciano;Favero, Gabriele;Mazzei, Franco
本文介绍了一种新型的基于表面等离子体共振技术和电化学法的生物传感器，用于检测维生素D(25OHD)。25-羟基维生素D(25OHD)是避免骨骼损伤和各种病理状态的重要因素，并评价其在临床上的潜在应用。本文介绍了利用亲和力生物传感器检测维生素D的不同方法：首先，基于SPR信号转导的免疫传感器实现了直接测定维生素D的免疫传感器，检测限为2微克/毫升。为了提高灵敏度，用金纳米颗粒(AuNPs)修饰维生素D：25OHD与AuNPs的结合决定了SPR信号的放大，使LOD降至1微克/毫升，使灵敏度翻了一番。最后，基于维生素D与2，2，4-三唑啉-3，5-二酮(FMTAD)的反应，建立了一种电化学传感器：衍生化后，25OHD的检测范围为20~200 ng/ml，检出限为10 ng/ml，满足了维生素D检测的要求；此外，由于使用了丝网印刷电极，这为传感器小型化和开发便携式且易于自动化的护理点测试设备提供了可能性。与耗费时间和试剂、需要放射性化合物、前处理程序和昂贵仪器的传统方法相比，拟议的设备提供了改进。(C)2012爱思唯尔B.V.保留所有权利。
This work has been focused on the development of novel optical (Surface Plasmon Resonance) and electrochemical based biosensors for the determination of 25-OH vitamin D (25OHD) which is an important factor involved in avoiding both skeletal damage and a variety of pathological conditions, and to evaluate their potential use in clinical practice.Different approaches to the determination of vitamin D using affinity based biosensors, are described herein; firstly, an immunosensor based on SPR transduction was realized for direct determination of vitamin D, obtaining a LOD of 2 mu g/ml which unfortunately is too far from the needs in clinical analysis. In order to enhance the sensitivity, the vitamin D was modified with gold nanoparticles (AuNPs): the binding of 25OHD with AuNPs determines the amplification of SPR signal, allowing to lower the LOD down to 1 mu g/ml, doubling the sensitivity. An alternative SPR method, based on the indirect determination of vitamin D by means of Vitamin D Binding Protein (VDBP), led to a further sensitivity increase reaching a LOD of 45 ng/ml which is really close to the fixed accomplishment.Finally, an electrochemical transduced biosensor has been realized, based on the reaction of vitamin D with 4-ferrocenylmethyl-1,2,4-triazoline-3,5-dione (FMTAD): once derivatized, the determination of 25OHD was possible in the range 20-200 ng/ml with a LOD of 10 ng/ml. The latter proposed system fits the requirement of determining vitamin D in a concentration range which is of significance for clinical applications; moreover, since a screen printed electrode has been used, this opens the possibility to miniaturize the sensor and developing a portable and easy-to-automate point-of-care testing device. The proposed devices provide an improvement with respect to traditional methods that are time and reagents consuming and require radioactive compounds, pretreatment procedures and expensive instrumentation. (C) 2012 Elsevier B.V. All rights reserved.