Quaternary complexes composed of plasmid DNA/protamine/fish sperm DNA/stearic acid grafted chitosan oligosaccharide micelles for gene delivery.
Quaternary complexes composed of plasmid DNA/protamine/fish sperm DNA/stearic acid grafted chitosan oligosaccharide micelles for gene delivery.
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DOI：
10.1016/j.ijbiomac.2010.10.011
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发表时间：
2011
影响因子：
8.2
通讯作者：
Yongzhong Du;P. Lu;Hong Yuan;Jian-ping Zhou;F. Hu
中科院分区：
文献类型：
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作者：
Yongzhong Du;P. Lu;Hong Yuan;Jian-ping Zhou;F. Hu
制备了具有质粒DNA、鱼精蛋白、鱼精DNA浓缩核和硬脂酸接枝壳寡糖(CSO-SA)壳的四元复合物。 CSO-SA可以在水溶液中自组装形成纳米尺寸的胶束，并表现出优异的肿瘤细胞内化能力。动态光散射（DLS）测量和透射静电显微镜（TEM）图像显示，四元复合物呈球形，数均直径约为25nm，并且四元复合物的尺寸小于CSO-SA胶束和CSO-SA胶束/质粒DNA二元复合物。四元复合物对HEK293和MCF-7细胞的转染效率随着孵育时间的延长而增加，并且显着高于CSO-SA胶束/质粒DNA二元复合物。流式细胞仪检测96h后四元复合物对HEK293和MCF-7细胞的最佳转染效率分别为23.82%和41.43%。而Lipofectamine™ 2000在96小时后对HEK293和MCF-7细胞的转染效率分别为32.45%和33.23%。荧光素酶活性测定数据表明,质粒DNA:鱼精子DNA:鱼精蛋白:CSO-SA的最佳比例为1:1:5:5。结果表明，本发明的四元复合物是潜在的非病毒基因传递系统。
Quaternary complexes with condensed core of plasmid DNA, protamine, fish sperm DNA and shell of stearic acid grafted chitosan oligosaccharide (CSO-SA), were prepared. The CSO-SA could self-assemble to form nano-sized micelles in aqueous solution and demonstrated excellent internalization ability of tumor cells. Dynamic light scattering (DLS) measurement and transmission electrostatic microscope (TEM) images showed that quaternary complexes had spherical shape with about 25nm number average diameter, and the size of quaternary complexes was smaller than that of CSO-SA micelles and CSO-SA micelles/plasmid DNA binary complexes. The transfection efficiencies of quaternary complexes on HEK293 and MCF-7 cells increased with incubation time, and were significantly higher than that of CSO-SA micelles/plasmid DNA binary complexes. The optimal transfection efficiency of quaternary complexes on HEK293 and MCF-7 cells measured by flow cytometer after 96h was 23.82% and 41.43%, respectively. Whereas, the transfection efficiency of Lipofectamine™ 2000 on HEK293 and MCF-7 cells after 96h was 32.45% and 33.23%, respectively. The data of luciferease activity measurement showed that the optimal ratio of plasmid DNA:fish sperm DNA:protamine:CSO-SA was 1:1:5:5. The results indicated that the present quaternary complexes were potential non-viral gene delivery system.