Sequence, secondary structure and phylogenetic analyses of the ribosomal internal transcribed spacer 2 (ITS2) in the Timarcha leaf beetles (Coleoptera: Chrysomelidae)
Sequence, secondary structure and phylogenetic analyses of the ribosomal internal transcribed spacer 2 (ITS2) in the Timarcha leaf beetles (Coleoptera: Chrysomelidae)
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DOI：
10.1046/j.1365-2583.2000.00223.x
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发表时间：
2000-12-01
影响因子：
2.6
通讯作者：
Petitpierre, E
中科院分区：
文献类型：
--
作者：
Gómez-Zurita, J;Juan, C;Petitpierre, E
本文报道了34种47份Timarcha属叶甲的rDNA内转录间隔区2（ITS 2）序列。Timarcha ITS 2（平均523 bp）与其他节肢动物近缘种（Chrysolina，Diabrotica和Bruchus）共享一些序列特征，但与任何其他节肢动物ITS 2序列没有明显的相似性。种间差异在0.002-0.166之间，亚属间差异在0.124-0.206之间。二级结构与脊椎动物和酵母提出的四域模型一致，但与双翅目动物提出的模型不同。ITS 2数据的系统发育分析证实了先前基于线粒体序列的研究结果，作为Metallotimarcha亚属的基础性和Timarchostoma亚属的系统发育支持的情况下。
Internal transcribed spacer 2 (ITS2) sequences of the nuclear rDNA in forty-seven specimens (thirty-four species) of the leaf beetle genus Timarcha have been studied. Timarcha ITS2 (523 bp on average) share some sequence features with other Chrysomeloidea relatives (Chrysolina, Diabrotica and Bruchus) but have no clear similarity with any other arthropod ITS2 sequences. Interspecific divergences are in the range 0.002-0.166, and 0.124-0.206 in the comparisons between subgenera. No evidence of intragenomic divergent ITS2 sequences has been found. Secondary structures are concordant with the four-domain model proposed for vertebrates and yeast, but differs from those proposed for dipterans. Phylogenetic analysis of the ITS2 data confirms the results of a previous study based on mitochondrial sequences, as the basality of the Metallotimarcha subgenus and the absence of phylogenetic support for the Timarchostoma subgenus.