Stage-specific proteins and glycoproteins of plasmodium falciparum: identification of antigens unique to schizonts and merozoites.
Stage-specific proteins and glycoproteins of plasmodium falciparum: identification of antigens unique to schizonts and merozoites.
复制标题
恶性疟原虫的阶段特异性蛋白质和糖蛋白：裂殖体和裂殖子特有抗原的鉴定。
DOI：
10.1073/pnas.77.6.3695
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发表时间：
1980
影响因子：
11.1
通讯作者：
A. Kilejian
中科院分区：
文献类型：
--
作者：
A. Kilejian
建立高度同步的恶性疟原虫培养，使鉴定阶段特异性蛋白、糖蛋白和抗原成为可能。比较环、滋养体、成熟分裂体和分裂子的代谢标记成分表明，环或滋养体所特有的主要蛋白质或糖蛋白缺失。当分裂体和分裂子体特异性蛋白质和糖蛋白出现时，新的合成活性爆发。除了先前被证明与宿主红细胞膜上的突起有关的旋钮蛋白外，还在分裂体的表面膜上发现了一种主要的寄生虫来源的糖蛋白。对不同发育阶段抗原的分析表明，体外抑制寄生虫生长的免疫血清主要与裂殖子和裂殖子相关抗原反应。
Establishment of highly synchronized cultures of Plasmodium falciparum enabled identification of stage-specific proteins, glycoproteins, and antigens. Comparison of metabolically labeled constituents of rings, trophozoites, mature schizonts, and merozoites indicated the absence of major proteins or glycorproteins unique to rings or trophozoites. A burst of new synthetic activity occurred during schizogony when several schizont- and merozoite-specific proteins and glycoproteins became apparent. In addition to the knob protein, which was previously shown to be associated with protrusions on the host erythrocyte membrane, a major glycoprotein of parasite origin was identified on the surface membrane of schizonts. Analysis of antigens solubilized from different developmental stages indicated that immune sera, which inhibit growth of parasites in vitro, react mainly with merozoite- and schizont-associated antigens.