Stereo- and biochemical profiles of the 5-6- and 6-6-junction isomers of α-D-mannopyranosyl [60] fullerenes
Stereo- and biochemical profiles of the 5-6- and 6-6-junction isomers of α-D-mannopyranosyl [60] fullerenes
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DOI：
10.1002/cbdv.200490106
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Kobayashi, K
中科院分区：
文献类型：
--
作者：
Nishida, Y;Mizuno, A;Kobayashi, K
用圆二色谱(CD)、氚标记、核磁共振氢谱、分子动力学(MD)计算和凝集素结合实验研究了U-D-甘露糖基[60]富勒烯的5-6-和6-6-结异构体。O-乙酰化衍生物的圆二色谱可以清楚地区分异构体，而H-1-核磁共振谱则结合氚标记和分子动力学计算，揭示了每个乙酰化异构体在氯仿溶液中的独特构象和分子构型。脱保护的5-6-和6-6-异构体在水溶液中形成胶体悬浮液，对刀豆蛋白A诱导的凝集素诱导的HEM凝集具有显著的阻断活性。
The 5-6- and 6-6-junction isomers Of U-D-mannopyranosyl [60]fullerene were studied by means of circular dichroism (CD), deuterium labeling, H-1-NMR, molecular-dynamics (MD) calculations, and a lectin-binding assay. The CD spectra of the O-acetylated derivatives allowed clear discrimination of the isomers, while the H-1-NMR spectra, with assistance from deuterium labeling and MD calculations, served to disclose the unique conformation and molecular geometry of each acetylated isomer in chloroform solution. The deprotected 5-6-and 6-6-isomers, which gave colloidal suspensions in aqueous mixtures, displayed marked activity in blocking lectin-induced hem agglutination by concanavalin A.