Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array
Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array
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DOI：
10.1117/1.1781668
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发表时间：
2004-09-01
影响因子：
3.5
通讯作者：
Rigler, R
中科院分区：
文献类型：
--
作者：
Gösch, M;Serov, A;Rigler, R
我们提出了一个完全集成的互补金属氧化物半导体（CMOS）单光子2 × 2探测器阵列的并行单分子探测（SMD）和荧光相关光谱（FCS）的实验。多焦激发是用衍射光学元件（DOE）实现的。特别强调的是整个系统的并行化。研究了新型单光子CMOS探测器的性能，并通过测量不同浓度的自由扩散分子，将其与最先进的单光子探测模块[具有主动猝灭雪崩光电二极管（APD）]进行了比较。尽管与最先进的单光子检测模块相比，CMOS检测器的检测效率低了一个数量级，但我们实现了单分子灵敏度并可靠地确定分子浓度。此外，CMOS检测器的性能，用于确定的分数的缓慢扩散的分子在引物溶液（双组分分析）的证明。这种新技术的高通量共焦检测为基础的系统的潜力进行了讨论。(C)2004年，美国光电仪器工程师学会。
We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2 X 2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed. (C) 2004 Society of Photo-Optical Instrumentation Engineers.