Aminoguanidine delays the replicative senescence of human diploid fibroblasts
Aminoguanidine delays the replicative senescence of human diploid fibroblasts
复制标题
氨基胍延缓人二倍体成纤维细胞的复制衰老
作者：
王培昌
目的研究氨基胍（2-AG）对人二倍体成纤维细胞（2BS）复制性衰老的影响。方法观察衰老表型、传代次数、细胞周期、细胞增殖潜能、晚期糖基化终产物（AGEs）水平和衰老相关β-半乳糖苷酶阳性细胞率结果2-AG作用2BS细胞后，细胞形态保持不变，细胞存活期延长至少19-20代，细胞增殖能力明显降低，细胞周期延长至少19-20代，细胞周期延长10 ~ 20代AG培养84 h后，细胞增殖率增加36%~ 40%，S期细胞比例是对照组的2倍，晚期传代细胞AGEs水平和SA-β-Gal阳性细胞率明显低于晚期传代对照组，结论2-AG可延缓2BS细胞的复制衰老。
Objective To study the effect of aminoguanidine (2-AG) on the replicative senescence of human diploid fibroblasts (2BS).Methods The changes of senescent phenotype, passages,cell cycle,the potential of cell proliferation,the levels of advanced glycation end-products (AGEs) and the positive cell rates of senescence-associated β-galactosidase (SA-β-Gal) of AG-effected 2BS cells were detected and analyzed.Results 2-AG maintained unaltered cell morphology and increased life span of 2BS by at least 19-20 passages.The proliferation potential of 2BS cells incubated with 2-AG for 84h was increased by 36%-40%.The percentage of the S-phase cells cultured by 2-AG was the double of that in the control cells.In addition,the levels of AGEs and the SA-β-Gal positive cell rates were significantly lower in late passage cells in sequenced multigeneration culture in 2-AG medium than those of late passage control cells,and similar to those of young cells.Conclusions 2-AG delays the replicative senescence of 2BS cells.