Electrostatic control of the membrane targeting of C2 domains
Electrostatic control of the membrane targeting of C2 domains
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DOI：
10.1016/s1097-2765(01)00426-9
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发表时间：
2002-01-01
期刊：
影响因子：
16
通讯作者：
Honig, B
中科院分区：
文献类型：
--
作者：
Murray, D;Honig, B
许多参与信号转导和囊泡运输的蛋白质都含有 C2 结构域，其膜结合通常受钙调节。这里，有限差分泊松-玻尔兹曼计算用于描述已知结构的 C2 域与磷脂膜之间的静电相互作用。结果解释了钙结合如何驱动一些 C2 结构域与带负电的膜的结合，以及其他 C2 结构域与中性、两性离子膜的结合。非特异性静电相互作用被证明是许多已知结构的 C2 结构域的一般特征，包括 PTEN 肿瘤抑制因子的钙独立 C2 结构域。
Many proteins involved in signal transduction and vesicle trafficking contain C2 domains whose membrane association is often regulated by calcium. Here, finite-difference Poisson-Boltzmann calculations are used to describe the electrostatic interactions between C2 domains of known structure and phospholipid membranes. The results explain how calcium binding can drive the association of some C2 domains to negatively charged membranes and others to neutral, zwitterionic membranes. Nonspecific electrostatic interactions are shown to be a general feature of many C2 domains of known structure, including the calcium-independent C2 domain of the PTEN tumor suppressor.