Superdomains in the protein structure hierarchy: The case of PTP-C2
Superdomains in the protein structure hierarchy: The case of PTP-C2
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DOI：
10.1002/pro.2664
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发表时间：
2015-05-01
期刊：
影响因子：
8
通讯作者：
Xue, Bin
中科院分区：
文献类型：
--
作者：
Haynie, Donald T.;Xue, Bin
超级结构域在这项工作中被独特地定义为不同蛋白质中不同球状结构域的保守组合。在超域假说测试中，对来自真菌、植物和动物的 25 种结构和功能不同的蛋白质的氨基酸序列进行了分析。每种蛋白质均包含一个蛋白质酪氨酸磷酸酶 (PTP) 结构域，后跟一个 C2 结构域。已鉴定出四种新的保守序列基序，一种位于 PTP 结构域，三种位于 C2 结构域。所有这些都有助于肿瘤抑制因子 PTEN 中的 PTP-C2 结构域界面，并且在 25 个序列中都比 PTP 特征基序 HCX3(K/R)XR 更保守。我们证明 PTP-C2 是在真菌、植物和动物界分化之前形成的。此处定义的超级域不适合通常的蛋白质结构分类系统。一个超级域的存在表明了其他超级域的存在。
Superdomain is uniquely defined in this work as a conserved combination of different globular domains in different proteins. The amino acid sequences of 25 structurally and functionally diverse proteins from fungi, plants, and animals have been analyzed in a test of the superdomain hypothesis. Each of the proteins contains a protein tyrosine phosphatase (PTP) domain followed by a C2 domain. Four novel conserved sequence motifs have been identified, one in the PTP domain and three in the C2 domain. All contribute to the PTP-C2 domain interface in PTEN, a tumor suppressor, and all are more conserved than the PTP signature motif, HCX3(K/R)XR, in the 25 sequences. We show that PTP-C2 was formed prior to the fungi, plant, and animal kingdom divergence. A superdomain as defined here does not fit the usual protein structure classification system. The demonstrated existence of one superdomain suggests the existence of others.