Centriole amplification by mother and daughter centrioles differs in multiciliated cells
Centriole amplification by mother and daughter centrioles differs in multiciliated cells
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DOI：
10.1038/nature13770
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发表时间：
2014-12-04
期刊：
影响因子：
64.8
通讯作者：
Meunier, Alice
中科院分区：
文献类型：
--
作者：
Al Jord, Adel;Lemaitre, Anne-Iris;Meunier, Alice
在周期细胞中，半保守的中心体复制产生了由母体中心粒和新形成的子代中心粒组成的中心体（1）。两个中心粒在形成新中心粒的能力上被认为是等同的，并且它们的对称复制对于细胞分裂稳态是至关重要的（2，4）。多纤毛细胞不使用原型复制程序，而是形成一百多个中心粒，这些中心粒是运动纤毛生长和有效推进生理液体所必需的（5）。这些新的中心粒中的大多数被认为是从头出现的，也就是说，独立于中心体，围绕电子致密的结构，称为后体（5，9）。它们的来源仍然不明。使用现场成像结合相关的超分辨率光学和电子显微镜，我们表明，所有新的中心粒来自预先存在的祖细胞中心体通过多轮的原中心粒播种。此外，我们确定，只有女儿中心体中心粒有助于后体的形成，从而超过百分之九十的最终中心粒人口。当研究纤毛相关疾病和在循环细胞中观察到的病理性中心粒扩增以及与小头畸形和癌症相关时，这种意想不到的中心粒不对称性赠款了新的视角（2- 4，10）。
The semi-conservative centrosome duplication in cycling cells gives rise to a centrosome composed of a mother and a newly formed daughter centriole(1). Both centrioles are regarded as equivalent in their ability to form new centrioles and their symmetric duplication is crucial for cell division homeostasis(2,4). Multiciliated cells do not use the archetypal duplication program and instead form more than a hundred centrioles that are required for the growth of motile cilia and the efficient propelling of physiological fluids(5). The majority of these new centrioles are thought to appear de novo, that is, independently from the centrosome, around electron-dense structures called deuterosomes(5,9). Their origin remains unknown. Using live imaging combined with correlative super-resolution light and electron microscopy, we show that all new centrioles derive from the pre-existing progenitor cell centrosome through multiple rounds of procentriole seeding. Moreover, we establish that only the daughter centrosomal centriole contributes to deuterosome formation, and thus to over ninety per cent of the final centriole population. This unexpected centriolar asymmetry grants new perspectives when studying cilia-related diseases and pathological centriole amplification observed in cycling cells and associated with microcephaly and cancer(2-4,10).