A neuroanatomical and physiological study of the non-image forming visual system of the cone-rod homeobox gene (Crx) knock out mouse.
A neuroanatomical and physiological study of the non-image forming visual system of the cone-rod homeobox gene (Crx) knock out mouse.
复制标题
锥杆同源盒基因（Crx）敲除小鼠非图像形成视觉系统的神经解剖学和生理学研究。
DOI：
10.1016/j.brainres.2010.04.066
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发表时间：
2010
期刊：
影响因子：
2.9
通讯作者：
Møller,Morten
中科院分区：
文献类型：
--
作者：
Rovsing,Louise;Rath,MartinF;Lund-Andersen,Casper;Klein,DavidC;Møller,Morten
在缺乏光感受器外段的视盲锥杆同源盒基因（Crx）敲除小鼠（Crx−/−）中，研究了非成像视觉系统的解剖和生理学。我们发现Crx−/−小鼠的视交叉上核（SCN）表现出与野生型小鼠相同的形态。此外，与野生型一样，SCN还含有血管活性肠肽、抗利尿激素和胃泌素释放肽免疫反应神经元。Crx - / -和野生型小鼠视网膜的顺行体内示踪显示，这两种动物的视网膜下丘脑向SCN和中央视神经通路的投射是相似的。对Crx−/−和野生型小鼠的运动活动和温度的遥测监测表明，Crx−/−和野生型小鼠均表现出24小时周期的昼夜节律，这种节律可以被光改变。然而，功率谱分析显示，Crx - / -小鼠的这两种节律都不如野生型小鼠强。Crx - / -小鼠中SCN和中央视觉通路的正常发育表明，视网膜外周光感受器向视网膜黑视素神经元或SCN的调节性输入可能是小鼠非成像系统正常功能所必需的。然而，Crx - / -小鼠SCN的变化也可能解释敲除小鼠和野生型小鼠之间观察到的昼夜节律差异。
The anatomy and physiology of the non-image forming visual system was investigated in a visually blind cone-rod homeobox gene (Crx) knock-out mouse (Crx−/−), which lacks the outer segments of the photoreceptors. We show that the suprachiasmatic nuclei (SCN) in the Crx−/−mouse exhibit morphology as in the wild type mouse. In addition, the SCN contain vasoactive intestinal peptide-, vasopressin-, and gastrin-releasing peptide-immunoreactive neurons as present in the wild type. Anterograde in vivo tracings from the retina of the Crx−/−and wild type mouse showed that the retinohypothalamic projection to the SCN and the central optic pathways were similar in both animals. Telemetric monitoring of the running activity and temperature revealed that both the Crx−/−and wild type mouse exhibited diurnal rhythms with a 24-h period, which could be phase changed by light. However, power spectral analysis revealed that both rhythms in the Crx−/−mouse were less robust than those in the wild type. The normal development of the SCN and the central visual pathways in the Crx−/−mouse suggests that a modulatory input from the photoreceptors in the peripheral retina to the retinal melanopsin neurons or the SCN may be necessary for a normal function of the non-image forming system of the mouse. However, a change in the SCN of the Crx−/−mouse might also explain the observed circadian differences between the knock out mouse and wild type mouse.