Conditions of embryo culture from days 5 to 7 of development alter the DNA methylome of the bovine fetus at day 86 of gestation
Conditions of embryo culture from days 5 to 7 of development alter the DNA methylome of the bovine fetus at day 86 of gestation
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DOI：
10.1007/s10815-019-01652-1
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发表时间：
2019-12
影响因子：
3.1
通讯作者：
Yahan Li;P. Tríbulo;M. Bakhtiarizadeh;L. Siqueira;Tieming Ji;R. M. Rivera;P. Hansen
中科院分区：
文献类型：
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作者：
Yahan Li;P. Tríbulo;M. Bakhtiarizadeh;L. Siqueira;Tieming Ji;R. M. Rivera;P. Hansen
PurposeWe测试是否在体外生产（IVP）的胎儿肝脏和骨骼肌中的DNA甲基化的变化，如果暴露于培养的胚胎集落刺激因子2（CSF2）改变DNA methylation.MethodsFemale胎儿产生人工授精或转移的IVP胚胎。在受精后第5至7天用CSF 2或媒介物处理胚胎。胎儿肝脏和骨骼肌中的DNA甲基化通过基于亚硫酸氢盐后衔接子标记的测序来确定。CpG位点的转录起始位点上游500 bp的启动子区域的50 bp的窗口中的DNA甲基化的程度进行了比较treatment.ResultsFor肝，有12个基因（6%的分析），其中DNA甲基化受治疗的影响，与一个50 bp的窗口，每个基因受治疗。对于肌肉，DNA甲基化程度受32个窗口（占分析的总窗口的19%）的处理影响，代表28个不同的基因（占分析基因的23%）。对于19的28个基因在肌肉中，DNA甲基化的最大偏差是CSF2 group.ConclusionResults是一致的甲基化组的改变是IVP的机制之一，可以导致胎儿发育和产后功能的改变在由此产生的后代。此外，结果表明，母体来源的细胞信号分子可以调节DNA甲基化的模式。
PurposeWe tested whether in vitro production (IVP) causes changes in DNA methylation in fetal liver and skeletal muscle and if exposure of cultured embryos to colony-stimulating factor 2 (CSF2) alters DNA methylation.MethodsFemale fetuses were produced by artificial insemination or transfer of an IVP embryo. Embryos were treated from days 5 to 7 after fertilization with CSF2 or vehicle. DNA methylation in fetal liver and skeletal muscle was determined by post-bisulfite adaptor tagging-based sequencing. The degree of DNA methylation for CpG sites in 50-bp windows of the promoter region 500 bp upstream of the transcriptional start site was compared between treatments.ResultsFor liver, there were 12 genes (6% of those analyzed) in which DNA methylation was affected by treatment, with one 50-bp window per gene affected by treatment. For muscle, the degree of DNA methylation was affected by treatment for 32 windows (19% of the total windows analyzed) representing 28 distinct genes (23% of analyzed genes). For 19 of the 28 genes in muscle, the greatest deviation in DNA methylation was for the CSF2 group.ConclusionResults are consistent with alterations in the methylome being one of the mechanisms by which IVP can result in altered fetal development and postnatal function in the resultant offspring. In addition, results indicate that maternally derived cell-signaling molecules can regulate the pattern of DNA methylation.