Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs.
Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs.
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DOI：
10.1262/jrd.10-200e
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
M. Nakai;N. Kashiwazaki;J. Ito;N. Maedomari;M. Ozawa;M. Shino;J. Noguchi;H. Kaneko;K. Kikuchi
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文献类型：
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作者：
M. Nakai;N. Kashiwazaki;J. Ito;N. Maedomari;M. Ozawa;M. Shino;J. Noguchi;H. Kaneko;K. Kikuchi
在卵胞浆内单精子注射（ICSI）技术中，使用玻璃移液管直接将精子注射到卵浆中。卵胞浆内单精子注射的受精生理被认为与自然受精有很大不同。不同的受精机制可能是导致ICSI结果不同的原因。本文主要从精子膜、细胞核和DNA完整性等方面探讨了ICSI过程中精子膜的状态对受精和胚胎发育的影响。本文还介绍了猪卵胞浆内单精子注射新技术的应用实例。
In intracytoplasmic sperm injection (ICSI) technique, a sperm was injected into ooplasm directly using a glass pipette. The fertilization physiology in ICSI is considered quite different from that of the natural fertilization. The different mechanisms for fertilization may be the causes of various results in ICSI. In this paper, we focus on the state of sperm membranes, nuclear or DNA integrity during ICSI procedure and discuss the influence of these factors on fertilization and embryonic development. We also introduce some examples in application of ICSI for new technologies in pigs.