Genome-wide screening for components of small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Genome-wide screening for components of small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
复制标题
对褐飞虱 Nilaparvata lugens（半翅目：飞虱科）中小干扰 RNA (siRNA) 和微小 RNA (miRNA) 途径成分进行全基因组筛选
DOI：
10.1111/imb.12051
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发表时间：
2013-12-01
影响因子：
2.6
通讯作者：
Zhang, C-X
中科院分区：
文献类型：
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作者：
Xu, H-J;Chen, T.;Zhang, C-X
褐飞虱（Nilaparvata lugens）是亚洲主要的水稻害虫，大量证据表明该物种对RNA干扰（RNAi）敏感，但RNAi和亲本RNAi的机制尚未确定。我们全面调查了小干扰RNA（siRNA）和micro-RNA（miRNA）途径中涉及的核心基因的剧目在BPH通过比较其新组装的转录组和基因组与果蝇，赤拟谷盗和秀丽隐杆线虫。我们的分析表明，BPH具有一个drosha和两个Dicer（dcr）基因，三个dsRNA结合基序蛋白基因，两个Argonaute（ago）基因，两个Eri-1-like基因（eri-1）和一个Sid-1-like基因（sid-1）。此外，我们还首次报道了亲本RNAi可能在该物种中发生，并且外源dsRNA引发的系统性RNAi的高效率需要siRNA途径和Sid-1的参与。此外，我们的研究结果还表明，miRNA途径参与了BPH的变态作为消耗的ago 1或dcr 1基因严重受损的蜕皮。褐飞虱是研究半变态昆虫系统性RNAi分子机制的良好模式系统，RNAi技术在农业生产中具有广阔的应用前景。
The brown planthopper (BPH), Nilaparvata lugens, is a major rice pest in Asia, and accumulated evidence indicates that this species is susceptible to RNA interference (RNAi); however, the mechanism underlying RNAi and parental RNAi has not yet been determined. We comprehensively investigated the repertoire of core genes involved in small interfering RNA (siRNA) and micro-RNA (miRNA) pathways in the BPH by comparing its newly assembled transcriptome and genome with those of Drosophila melanogaster, Tribolium castaneum and Caenorhabditis elegans. Our analysis showed that the BPH possesses one drosha and two Dicer (dcr) genes, three dsRNA-binding motif protein genes, two Argonaute (ago) genes, two Eri-1-like genes (eri-1), and a Sid-1-like gene (sid-1). Additionally, we report for first time that parental RNAi might occur in this species, and siRNA pathway and Sid-1 were required for high efficiency of systemic RNAi triggered by exogenous dsRNA. Furthermore, our results also demonstrated that the miRNA pathway was involved in BPH metamorphosis as depletion of the ago1 or dcr1 gene severely impaired ecdysis. The BPH might be a good model system to study the molecular mechanism of systemic RNAi in hemimetabolous insects, and RNAi has potential to be developed to control this pest in agricultural settings.