Molecular epidemiology of bluetongue virus serotype 4 isolated in the Mediterranean Basin between 1979 and 2004
Molecular epidemiology of bluetongue virus serotype 4 isolated in the Mediterranean Basin between 1979 and 2004
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DOI：
10.1016/j.virusres.2007.01.002
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发表时间：
2007-05-01
期刊：
影响因子：
5
通讯作者：
Zientara, Stephan
中科院分区：
文献类型：
--
作者：
Breard, Emmanuel;Sailleau, Corinne;Zientara, Stephan
测定了分别编码病毒蛋白（VP）和非结构蛋白（NS）-VP2、VP7、NS2、VP6 和 NS3/NS3A 的基因组片段 2、7、8、9 和 10 的核苷酸序列，并比较了 1979 年至 2004 年间在地中海盆地分离的 10 株蓝舌病病毒（BTV）血清型 4 和南非减毒 BTV 4疫苗株。 1979年、1999年和2000年在希腊分离的BTV 4菌株产生的序列数据表明，它们具有共同的起源，但与2003年以来在西地中海盆地（意大利、摩洛哥、西班牙和科西嘉岛）分离的BTV 4菌株的谱系不同。每个谱系内的 BTV 4 菌株的核苷酸和推导的氨基酸 (aa) 序列彼此相同，无论分离年份或地理位置如何。尽管土耳其和希腊菌株的 VP2 序列高度相似，但 VP6、VP7 和 NS2 蛋白存在足够的差异，表明土耳其 BTV 4 属于第三个谱系。减毒 BTV 4 疫苗株和野外株的 NS3 序列比对显示有 13 个氨基酸替换，这些替换可能单独或一起导致减毒，从而决定病毒的毒力。 (c) 2007 Elsevier B.V. 保留所有权利。
The nucleotide sequences of genome segments 2, 7, 8, 9 and 10, coding for viral proteins (VP) and non-structural proteins (NS)-VP2, VP7, NS2, VP6 and NS3/NS3A, respectively, were determined and compared for 10 strains of bluetongue virus (BTV) serotype 4 isolated in the Mediterranean Basin between 1979 and 2004, and the South African attenuated BTV 4 vaccine strain. The sequence data generated for the BTV 4 strains isolated in Greece in 1979, 1999 and 2000 showed that they had a common origin but were distinct from the lineage of the BTV 4 strains isolated from 2003 onward in the western Mediterranean Basin (Italy, Morocco, Spain and Corsica). The nucleotide and deduced amino acid (aa) sequences of the BTV 4 strains within each lineage were identical to each other, irrespective of the year of isolation or the geographical location. Although the sequence of VP2 from the Turkish and Greek strains were highly similar, there were sufficient differences in the VP6, VP7 and NS2 proteins to suggest that the Turkish BTV 4 belongs to a third lineage. Alignment of the NS3 sequences from the attenuated BTV 4 vaccine strain and the field strains showed 13 aa substitutions, which may, either singularly or together, be responsible for attenuation and hence determining the virulence of the virus. (c) 2007 Elsevier B.V. All rights reserved.