DNA Barcoding of Mealybugs (Hemiptera: Coccoidea: Pseudococcidae) From Mainland China
DNA Barcoding of Mealybugs (Hemiptera: Coccoidea: Pseudococcidae) From Mainland China
复制标题
中国大陆粉虱（半翅目：球虫总科：拟球虫科）的 DNA 条形码
DOI：
10.1093/aesa/saw009
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发表时间：
2016-05-01
影响因子：
2.3
通讯作者：
Wu, San-An
中科院分区：
文献类型：
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作者：
Wang, Xu-Bo;Zhang, Jiang-Tao;Wu, San-An
粉蚧（半翅目：粉蚧科）是一个以植物汁液为食的昆虫类群;许多是世界范围内观赏植物和作物的主要害虫。粉蚧形态鉴定的困难表明需要一种快速有效的鉴定方法，如DNA条形码，以协助形态分类。本研究采用最佳匹配（best close match，BLOG）、邻接树（Neighbor-Joining，NJ）、LOGic公式条形码（Barcoding with LOGic formulations，BLOG）、泊松树（Poisson Tree Process，PTP）等多种方法，对线粒体细胞色素c氧化酶I（Cytochrome c Oxidase I，COI）和核糖体大亚基基因（Large ribosomal subunit gene，28 S）这两种分子标记在我国常见的54种粉蚧物种鉴定中的有效性进行了研究。从246个个体中回收了206个COI条形码（47个物种）和242个28 S序列（53个物种）。在COI和28 S数据集中，除柑橘动球菌和小动球菌外，所有方法均明确鉴定了物种。基于COI序列的PTP分析比形态学鉴定产生更多的方孔安东尼娅、沼泽Atrococcus paludinus和蚁球菌属的推定种。在这三种情况下，序列的At.本研究证实了COI基因和28 S基因在粉蚧快速鉴定中的实用性，所建立的条形码文库将为我国粉蚧害虫的防治提供有效的鉴定体系。
Mealybugs (Hemiptera: Pseudococcidae) are an insect group that feeds on plant sap; many are major pests of ornamental plants and crops worldwide. The difficulty of morphological identification of mealybugs points to a need for a rapid and effective identification method, like DNA barcoding, to assist morphological taxonomy. Here, we employed diverse methods (best close match [BCM], Neighbor-Joining [NJ] tree, Barcoding with LOGic formulas [BLOG], Poisson Tree Process [PTP] Species Delimitation Method) to test the efficiency of two molecular markers (mitochondrial cytochrome c oxidase I [COI] and large ribosomal subunit gene [28S]) that could be used for species identification of 54 mealybug species that commonly occur in China. Two hundred six COI barcodes (47 species) and 242 28S sequences (53 species) were recovered from 246 individuals. In both the COI and 28S data sets, species except for Planococcus citri and P. minor were unambiguously identified by all the methods. The PTP analysis based on COI sequences generated more putative species in Antonina tesquorum , Atrococcus paludinus , and Formicococcus sp. than morphological identification. Among these three cases, the sequences of At. paludinus showed 3.55% variation at the 28S locus, possibly reflecting cryptic diversity in this taxon. Our study corroborates the utility of the COI and 28S genes in the rapid identification of mealybugs, and the barcode library we provide will create an effective identification system for mealybug pest management in China.